Abstract | OBJECTIVE: METHODS: Twenty-four female NOD mice at onset of diabetes and aged 17-20 weeks old were randomly divided into four groups. Mouse 1, 2 and 3 groups were intraperitoneally injected 0.1 ml of Ad-mIGF-1, Ad-mIL-10, and combined Ad-mIGF-1 and Ad-mIL-10, respectively. Mouse 4 group were used as diabetes control. In addition, six age- and sex-matched non-diabetic NOD mice were intraperitoneally injected 0.1 ml of PBS and assigned 5 group as normal controls. All mice were weekly monitored for body weight, urine glucose and blood glycose, and sacrificed 3 weeks after injection. Their serum levels of IL-10, IGF-1, IFN-γ, IL-4 and C-peptide were measured and the degree of insulitis and the local expression of IGF-1 and IL-10 gene were observed. RESULTS: 1) IL-10 and IGF-1 levels in serum and pancreas were enhanced in 1, 2, and 3 groups; 2) serum INF-γ level was decreased while serum IL-10 and IL-4 levels were increased in 1, 2 and 3 groups, and these alterations were more significant in 3 group than 1 and 2 groups (P<0.01); 3) C-peptide level was not enhanced in 1 group, but significantly increased in 2 and 3 groups, and these increases were more significant in the latter (P<0.01); 4) Three weeks later, the body mass of mice in 2 and 3 groups decreased significantly (P<0.05). CONCLUSION: The administration of adenovirus vector mediated IL-10 and/or IGF-1 gene showed limited immune regulatory and protective effects on islet β-cells in NOD mice with T1D at early stage, and no significant reduction in insulitis, blood glucose and body weight.
|
Authors | Lijuan Zhang, Yanyan Chen, Cheng Li, Xiaojie Lin, Xiaoli Cheng, Tang Li |
Journal | PloS one
(PLoS One)
Vol. 9
Issue 3
Pg. e92616
( 2014)
ISSN: 1932-6203 [Electronic] United States |
PMID | 24663217
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Blood Glucose
- Interleukin-10
- Insulin-Like Growth Factor I
|
Topics |
- Adenoviridae
(genetics)
- Animals
- Blood Glucose
(metabolism)
- Body Weight
(genetics)
- Diabetes Mellitus, Type 1
(blood, genetics, pathology, therapy)
- Female
- Gene Expression Regulation
(genetics)
- Genetic Therapy
- Genetic Vectors
(genetics)
- Insulin-Like Growth Factor I
(genetics)
- Interleukin-10
(blood, genetics)
- Islets of Langerhans
(metabolism)
- Mice
- Mice, Inbred NOD
|