Induced cell death pathway post photodynamic therapy using a metallophthalocyanine photosensitizer in breast cancer cells.

Abstract

OBJECTIVE:

Zinc phthalocyanine (ZnPcSmix) was used as the photosensitizer (PS) in this study to investigate the cell death patterns as a result of photodynamic therapy (PDT) in a breast cancer cell line (MCF-7) in vitro using a 680 nm diode laser at a fluence of 5 J/cm(2).

BACKGROUND:

PDT is a noninvasive form of cancer therapy, successfully applied for the treatment of various cancer types.

METHODS:

Flow cytometry using Annexin V-fluorescein isothiocyanate (FITC), a cell death immunosorbent assay (ELISA), and gene expression analysis following ZnPcSmix mediated PDT were performed to determine the induced cell death pathways.

RESULTS:

The apoptotic cells abounded after the treatment, nuclear fragmentation was seen as oligonucleosomal degradation and increased expression of the B-cell lymphoma 2 (Bcl-2), DNA fragmentation factor alpha (DFFA1), and caspase 2 (CASP2) genes, indicated that apoptosis is the main induced mode of cell death.

CONCLUSIONS:

ZnPcSmix mediated PDT led to an apoptotic cell death pathway and the PS used showed its ability to stimulate and initiate programmed cell death.

• Authors: Ivan Mfouo-Tynga, Nicolette N Houreld, Heidi Abrahamse
• Journal: Photomedicine and laser surgery (Photomed Laser Surg) Vol. 32 Issue 4 Pg. 205-11 (Apr 2014) ISSN: 1557-8550 [Electronic] United States
• PMID: 24661060 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Indoles
• Isoindoles
• Organometallic Compounds
• Photosensitizing Agents
• Zinc Compounds
• Zn(II)-phthalocyanine

Topics

• Apoptosis
• Breast Neoplasms (drug therapy, pathology)
• Cell Death (physiology)
• Cells, Cultured
• Female
• Humans
• Indoles (therapeutic use)
• Isoindoles
• Organometallic Compounds (therapeutic use)
• Photochemotherapy (methods)
• Photosensitizing Agents (therapeutic use)
• Zinc Compounds