Abstract | AIMS: MAIN METHODS: Conventional Liu's staining is an auxiliary technique used in microscopy to enhance the contrast in microscopic images, aiding the observation of cell morphology. The MTT assay, flow cytometry of the sub-G1 analysis and the TUNEL assay were applied to estimate the apoptosis levels. RT-PCR and western blot methods were used to evaluate the key molecules conferring anti-cell-death properties. KEY FINDINGS: The effects of the tyrosine kinase inhibitor AG1024 were evaluated with regard to the regulation of BCR-ABL expression, inhibition of cell proliferation, and enhanced paclitaxel-induced apoptosis in BCR-ABL-expressing K562 cell lines. AG1024 downregulated the expression of BCR-ABL and anti-apoptosis factors, such as Bcl-2 and Bcl-xL, which were present in K562 cells. Moreover, the combination of AG1024 with paclitaxel inhibited cell proliferation and enhanced paclitaxel-induced apoptosis within 24h. SIGNIFICANCE:
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Authors | Hao-Yuan Cheng, Fu-Hsiang Ko |
Journal | Life sciences
(Life Sci)
Vol. 102
Issue 2
Pg. 118-26
(May 02 2014)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 24657894
(Publication Type: Journal Article)
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Copyright | Copyright © 2014 Elsevier Inc. All rights reserved. |
Chemical References |
- Antineoplastic Agents, Phytogenic
- Tyrphostins
- tyrphostin AG 1024
- Paclitaxel
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Topics |
- Antineoplastic Agents, Phytogenic
(administration & dosage)
- Apoptosis
(drug effects, physiology)
- Drug Delivery Systems
(methods)
- Drug Therapy, Combination
- Humans
- K562 Cells
- Leukemia, Myelogenous, Chronic, BCR-ABL Positive
(drug therapy, pathology)
- Paclitaxel
(administration & dosage)
- Tyrphostins
(administration & dosage)
- Up-Regulation
(drug effects, physiology)
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