Abstract | BACKGROUND:
Schistosomiasis remains a highly prevalent and serious parasitic disease. A major factor preventing its effective management is the scarcity of effective diagnostic tools. We did a genome-wide identification of diagnostic protein markers for schistosome infection and assessed their diagnostic validity in a field study. METHODS: We predicted putative secreted proteins of Schistosoma japonicum (SjSPs) and expressed them as glutathione S-transferase (GST)-fusion proteins. The fusion proteins were arrayed on glutathione (GSH)-immobilised microplates and screened with serum samples from patients with schistosomiasis diagnosed by the Kato-Katz method. We further assessed an identified protein marker for sensitivity and specificity, first in infected serum samples collected from Jiangxi and Hunan Provinces, China, and then through a field study, done in two villages located in a high schistosomiasis-endemic area of the southeast of China. FINDINGS: Of 204 recombinant proteins, 35 yielded seropositive reactions, eight showed strong immunoreactivity, and only one (SjSP-13) reacted to the entire panel of 14 archived samples. The reactivity of SjSP-13 to 476 serum samples showed 90·4% (95% CI 86·5-93·5) sensitivity and 98·9% (95% CI 95·9-99·9) specificity. Of 1371 residents enrolled in a field study from Dec 6, 2010, to June 23, 2011, only 74 individuals were identified as being egg-positive, whereas 465 were diagnosed as positive by the SjSP-13-based ELISA kit (rSP13-ELISA). Of the 394 individuals found egg-negative but rSP13-ELISA-positive, 363 (92·4%) were confirmed to be positive for schistosome infection by PCR detection of S japonicum SjR2 retrotransposon. INTERPRETATION: The application of this sensitive, specific, and affordable rSP13-ELISA method should help reduce schistosomiasis transmission through targeted treatment of individuals, particularly with low intensity infections, and therefore support schistosomiasis control and elimination strategies. FUNDING: National 973 project in China.
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Authors | Xindong Xu, Yuanbin Zhang, Dandan Lin, Jinjin Zhang, Jin Xu, Yue-Min Liu, Fei Hu, Xiaoxing Qing, Chaoming Xia, Weiqing Pan |
Journal | The Lancet. Infectious diseases
(Lancet Infect Dis)
Vol. 14
Issue 6
Pg. 489-97
(Jun 2014)
ISSN: 1474-4457 [Electronic] United States |
PMID | 24656567
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Ltd. All rights reserved. |
Chemical References |
- Anthelmintics
- Antibodies, Helminth
- Biomarkers
- Helminth Proteins
- Recombinant Fusion Proteins
- Praziquantel
- Glutathione Transferase
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Topics |
- Adolescent
- Adult
- Aged
- Animals
- Anthelmintics
(therapeutic use)
- Antibodies, Helminth
(blood)
- Biomarkers
(metabolism)
- Child
- Child, Preschool
- China
- Enzyme-Linked Immunosorbent Assay
(methods)
- Feces
(parasitology)
- Female
- Gene Expression
- Glutathione Transferase
(immunology)
- Helminth Proteins
(genetics, immunology)
- Humans
- Male
- Middle Aged
- Praziquantel
(therapeutic use)
- Prevalence
- Recombinant Fusion Proteins
(immunology)
- Schistosoma japonicum
(genetics, immunology, isolation & purification)
- Schistosomiasis japonica
(diagnosis, drug therapy, parasitology)
- Sensitivity and Specificity
- Young Adult
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