Abstract |
Notopterygium roots ( Qiang Huo) have been used in traditional Chinese medicine for treating colds, inflammatory diseases like rheumatoid arthritis, and as an analgesic. The anti-inflammatory activity of the roots of Notopterygium incisum has been evaluated by testing the inhibitory activity on nitric oxide production by inducible nitric oxide synthase. The apparent authenticity of the sample was checked by DNA sequence comparison. Using activity-guided isolation, different compounds were isolated and structurally characterized by means of NMR and mass spectroscopy. Eight polyacetylenes could be identified and were tested on their inhibitory activity on nitric oxide production in RAW 264.7 mouse macrophages using the Griess assay. Different 3-hydroxy allyl polyacetylenes exhibited significant activity (IC50: 8-acetoxyfalcarinol, 20.1 µM; falcarindiol, 9.2 µM; 9-epoxyfalcarindiol, 8.8 µM; and crithmumdiol, 23.6 µM).
|
Authors | Martina Blunder, Xin Liu, Olaf Kunert, Nora Anna Winkler, Andreas Schinkovitz, Corinna Schmiderer, Johannes Novak, Rudolf Bauer |
Journal | Planta medica
(Planta Med)
Vol. 80
Issue 5
Pg. 415-8
(Mar 2014)
ISSN: 1439-0221 [Electronic] Germany |
PMID | 24652524
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Georg Thieme Verlag KG Stuttgart · New York. |
Chemical References |
- Polyynes
- Nitric Oxide
- Nitric Oxide Synthase Type II
|
Topics |
- Animals
- Apiaceae
(chemistry)
- Cell Line
- In Vitro Techniques
- Magnetic Resonance Spectroscopy
- Mass Spectrometry
- Mice
- Molecular Sequence Data
- Nitric Oxide
(biosynthesis)
- Nitric Oxide Synthase Type II
(metabolism)
- Plant Roots
(chemistry)
- Polyynes
(isolation & purification, pharmacology)
|