Abstract | OBJECTIVE: APPROACH AND RESULTS: Ldlr(-/-) mice were transplanted with ApoE(-/-)Cbs(-/-) or ApoE(-/-) BM followed by Western-type diet feeding. Compared with ApoE(-/-) BM-transplanted controls, ApoE(-/-)Cbs(-/-) BM-transplanted mice had reduced BM and splenic HSPC proliferation, fewer blood monocytes and neutrophils, and reduced macrophage content and area of early atherosclerotic lesions. More advanced lesions showed diminished macrophage and collagen content; however, lesion size was unchanged, reflecting an increase in necrotic core area, associated with a marked decrease in Abcg1 expression and increased macrophage apoptosis. Compared with wild-type mice, Western-type diet-fed ApoE(-/-) mice showed increased CBS expression on granulocyte macrophage colony-stimulating factor-producing innate response activator B cells and expansion of this population. ApoE(-/-)Cbs(-/-) BM-transplanted Ldlr(-/-) mice showed a marked decrease in innate response activator B cells compared with ApoE(-/-) BM-transplanted Ldlr(-/-) controls. CONCLUSIONS: Increased levels of CBS on HSPCs and splenic innate response activator B cells lead to expansion of these populations in ApoE(-/-) BM-transplanted Ldlr(-/-) mice, contributing to monocytosis and increased lesional macrophage content. However, in more advanced lesions, the CBS also has a role in atherosclerotic plaque stabilization.
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Authors | Mi Wang, Manikandan Subramanian, Sandra Abramowicz, Andrew J Murphy, Ayelet Gonen, Joseph Witztum, Carrie Welch, Ira Tabas, Marit Westerterp, Alan R Tall |
Journal | Arteriosclerosis, thrombosis, and vascular biology
(Arterioscler Thromb Vasc Biol)
Vol. 34
Issue 5
Pg. 976-84
(May 2014)
ISSN: 1524-4636 [Electronic] United States |
PMID | 24651678
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- ABCG1 protein, mouse
- ATP Binding Cassette Transporter, Subfamily G, Member 1
- ATP-Binding Cassette Transporters
- Apolipoproteins E
- Cytokine Receptor Common beta Subunit
- Lipoproteins
- Receptors, LDL
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Topics |
- ATP Binding Cassette Transporter, Subfamily G, Member 1
- ATP-Binding Cassette Transporters
(metabolism)
- Animals
- Aortic Diseases
(genetics, metabolism, pathology)
- Apolipoproteins E
(deficiency, genetics)
- Atherosclerosis
(genetics, metabolism, pathology)
- B-Lymphocytes
(metabolism, pathology)
- Bone Marrow Cells
(metabolism, pathology)
- Bone Marrow Transplantation
- Cell Differentiation
- Cell Proliferation
- Cytokine Receptor Common beta Subunit
(deficiency, genetics, metabolism)
- Disease Models, Animal
- Disease Progression
- Hematopoietic Stem Cells
(metabolism, pathology)
- Immunity, Innate
- Lipoproteins
(metabolism)
- Macrophages
(metabolism, pathology)
- Mice
- Mice, 129 Strain
- Mice, Inbred C57BL
- Mice, Knockout
- Monocytes
(metabolism, pathology)
- Necrosis
- Plaque, Atherosclerotic
- Receptors, LDL
(deficiency, genetics)
- Signal Transduction
- Spleen
(metabolism, pathology)
- Time Factors
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