Abstract | BACKGROUND AND OBJECTIVE: STUDY DESIGN/MATERIALS AND METHODS:
Gold nanoshell loaded rat macrophages either alone or combined with human FaDu squamous cells in hybrid monolayers were subjected to PTT, PDT, or a simultaneous combination of the two light treatments. Therapies were given concurrently employing two laser light sources of λ = 670 nm ( PDT) and λ = 810 nm (PTT), respectively. RESULTS: Significant uptake of gold nanospheres (AuNS) by rat alveolar macrophages was observed thus providing the rationale for their use as delivery vectors. Viability of the AuNS-loaded Ma was reduced to 35 and 12% of control values at an irradiance of 14 or 28 W/cm(2) administered over a 5 minute period respectively. No significant cytotoxicity was observed for empty Ma for similar PTT exposure. AlPcS2a mediated PDT at a fluence level of 0.25 J/cm(2) and PTT at 14 W/cm(2) irradiance had little effect on cell viability for the FaDu/Ma (ratio 2:1) hybrid monolayers. In contrast, combined treatment reduced the cell viability to less than 40% at these same laser power settings. CONCLUSIONS: The results of this study provide proof of concept for the use of macrophages as a delivery vector of AuNS for photothermal enhancement of the effects of PDT on squamous cell carcinoma. A significant synergy was demonstrated with combined PDT and PTT compared to each modality applied separately.
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Authors | Anthony J Trinidad, Seok Jin Hong, Qian Peng, Steen J Madsen, Henry Hirschberg |
Journal | Lasers in surgery and medicine
(Lasers Surg Med)
Vol. 46
Issue 4
Pg. 310-8
(Apr 2014)
ISSN: 1096-9101 [Electronic] United States |
PMID | 24648368
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 Wiley Periodicals, Inc. |
Chemical References |
- Antineoplastic Agents
- Indoles
- Organometallic Compounds
- Photosensitizing Agents
- aluminum phthalocyanine disulfonate
- Gold
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Topics |
- Animals
- Antineoplastic Agents
(therapeutic use)
- Carcinoma, Squamous Cell
(therapy)
- Cell Line, Tumor
- Combined Modality Therapy
- Drug Delivery Systems
(methods)
- Gold
(therapeutic use)
- Head and Neck Neoplasms
(therapy)
- Humans
- Hyperthermia, Induced
(methods)
- Indoles
(therapeutic use)
- Macrophages
- Nanoshells
(therapeutic use)
- Organometallic Compounds
(therapeutic use)
- Photochemotherapy
(methods)
- Photosensitizing Agents
(therapeutic use)
- Rats
- Squamous Cell Carcinoma of Head and Neck
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