Abstract | BACKGROUND: Institut Georges Lopez-1 preservation solution (IGL-1) is an emerging extracellular-type electrolyte solution, low in viscosity, containing polyethylene glycol 35 as a colloid. Although IGL-1 has shown beneficial outcomes in kidney and liver preservation, this pilot study is the first to evaluate the efficacy of IGL-1 in pancreas transplantation (PT) compared with the University of Wisconsin solution (UW). METHODS: Sixteen Landrace pigs underwent allogeneic PT with 16 hr of cold ischemia. Grafts were preserved with IGL-1 (n=8) or UW (n=8). No immunosuppression was administered. We analyzed graft function, the acute-phase response, and oxidative stress in the pancreatic graft monitoring membrane fluidity and lipid peroxidation. RESULTS: All eight grafts with IGL-1, but only six with UW, were functioning. Graft failures with UW resulted from graft thrombosis. There were no differences between the two solutions in the number of normoglycemic days (IGL-1: 11.5 ± 6.2 versus UW: 8.5 ± 4.4 days, P=0.1357), nor in lipid peroxidation during 16-hr cold ischemia (P=0.672), or reperfusion (P=0.185), but IGL-1 prevented changes in membrane fluidity after reperfusion when compared with UW (P=0.026). CONCLUSION: IGL-1 offered the same degree of safety and effectiveness as UW in our model of pig PT with 16 hr of cold ischemia.
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Authors | Francisco A García-Gil, Lorena Fuentes-Broto, Carlos D Albendea, María Trinidad Serrano, Joan Roselló-Catafau, Fermín Lampreave, Laura López-Pingarrón, Jorge Escartín, Joaquín Soria, Joaquín J Garcia, Laureano Fernández-Cruz |
Journal | Transplantation
(Transplantation)
Vol. 97
Issue 9
Pg. 901-7
(May 15 2014)
ISSN: 1534-6080 [Electronic] United States |
PMID | 24646772
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Colloids
- Electrolytes
- IGL-1 solution
- Insulin
- Organ Preservation Solutions
- University of Wisconsin-lactobionate solution
- Polyethylene Glycols
- Allopurinol
- Glutathione
- Adenosine
- Raffinose
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Topics |
- Adenosine
(chemistry)
- Allopurinol
(chemistry)
- Animals
- Colloids
(chemistry)
- Electrolytes
- Female
- Glutathione
(chemistry)
- Immunosuppression Therapy
- Insulin
(chemistry)
- Ischemia
- Kidney
(pathology)
- Lipid Peroxidation
- Liver
(pathology)
- Organ Preservation
(methods)
- Organ Preservation Solutions
(chemistry)
- Oxidative Stress
- Pancreas
(pathology)
- Pancreas Transplantation
(methods)
- Pilot Projects
- Polyethylene Glycols
(chemistry)
- Raffinose
(chemistry)
- Swine
- Time Factors
- Viscosity
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