[Evaluation of a rapid diagnostic test in the diagnosis of toxoplasmosis in pregnant women in Cotonou (Bénin)].

Abstract

The aim of the study was to evaluate the performance of the ImmunoComb® Toxo IgG and ImmunoComb® Toxo IgMassays (rapid diagnostic test) in the laboratory diagnosis of toxoplasmosis in pregnant women in Cotonou. We interviewed 266 pregnant women, who first answered an epidemiological questionnaire, and collected blood samples for measurement of IgG and IgM anti T. gondii antibodies with the ImmunoComb toxo assays and with the ARCHITECT CIMA method. The sensitivity, specificity, positive predictive values (PPV) and negative predictive values (NPV) were calculated to determine the performance of the rapid test. The seroprevalences of IgG against T. gondii by CIMA technique and rapid test were respectively 48.9% and 48.5%. The prevalence increased with age. Performances for IgG were: sensitivity 97%, specificity 100%, PPV 100%, NPV = 97.10%. For IgM, Sensitivity: 33.3% Specificity: 100%, PPV 100%, NPV = 99.2%. Seroprevalence obtained shows that about half of the study population is not immune against T. gondii and requires regular serological monitoring until delivery. According to these results, and given the needs of toxoplasmosis diagnosis on the field characterized by an important decrease of immunized women, this test may be recommended in the laboratory diagnosis of toxoplasmosis in peripheral levels of the health pyramid.

• Authors: A Ogouyèmi-Hounto, F Agbayahoun-Chokki, Y Sissinto Savi de Tove, B Biokou Bankole, V Adinsi de Souza, M Assogba, D Kinde-Gazard, A Massougbodji
• Journal: Bulletin de la Societe de pathologie exotique (1990) (Bull Soc Pathol Exot) Vol. 107 Issue 2 Pg. 85-9 (May 2014) ISSN: 1961-9049 [Electronic] France
• Vernacular Title: Évaluation d'un test de diagnostic rapide pour le diagnostic de la toxoplasmose chez les femmes enceintes à Cotonou (Bénin).
• PMID: 24639136 (Publication Type: Clinical Trial, Comparative Study, English Abstract, Journal Article)

Chemical References

• Antibodies, Protozoan
• Antigen-Antibody Complex
• Chromogenic Compounds
• Immunoglobulin G
• Immunoglobulin M
• Reagent Kits, Diagnostic

Topics

• Adolescent
• Adult
• Age Factors
• Antibodies, Protozoan (blood)
• Antigen-Antibody Complex (analysis)
• Benin (epidemiology)
• Chromogenic Compounds (analysis)
• Colorimetry (methods)
• Female
• Humans
• Immunoglobulin G (blood, immunology)
• Immunoglobulin M (blood, immunology)
• Luminescent Measurements
• Predictive Value of Tests
• Pregnancy
• Pregnancy Complications, Infectious (blood, diagnosis, epidemiology)
• Reagent Kits, Diagnostic
• Sensitivity and Specificity
• Seroepidemiologic Studies
• Surveys and Questionnaires
• Time Factors
• Toxoplasma (immunology)
• Toxoplasmosis (blood, diagnosis, epidemiology)
• Toxoplasmosis, Congenital (prevention & control)
• Young Adult