Anti-inflammatory potential of alpha-linolenic acid mediated through selective COX inhibition: computational and experimental data.

Abstract

The present work investigates the anti-inflammatory activity of alpha-linolenic acid (ALA) and linoleic acid (LA) using computational and experimental analysis. The binding affinity of ALA and LA was appraised for cyclooxygenase 1 (COX-1), cyclooxygenase 2 (COX-2), and 5-lipoxygenase (5-LOX) using AutoDock 4.2 and AutoDock Vina 1.1.2. Anti-inflammatory activity of ALA (2 and 4 ml/kg, i.p.) (55.65 % v/v) and LA (2 and 4 ml/kg, i.p.) (55 % v/v) was further assayed using the rat paw edema test against a variety of phlogistic agents including carrageenan, arachidonic acid, prostaglandin, and leukotriene, respectively. ALA (2 and 4 ml/kg, i.p.) and LA (2 and 4 ml/kg, i.p.) were further tested for their efficacy against complete Freund's adjuvant (CFA)-induced (0.05 ml) arthritis in albino rats. Following CFA-induced arthritis, ALA and LA were tested for their inhibitory proficiency against COX-1, COX-2, and 5-LOX in vitro. The present study commends that the anti-inflammatory potential of ALA could be attributed to COX inhibition, in particular, COX-2.

• Authors: Richa Anand, Gaurav Kaithwas
• Journal: Inflammation (Inflammation) Vol. 37 Issue 4 Pg. 1297-306 (Aug 2014) ISSN: 1573-2576 [Electronic] United States
• PMID: 24639012 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Cyclooxygenase Inhibitors
• Leukotrienes
• Lipoxygenase Inhibitors
• Prostaglandins
• alpha-Linolenic Acid
• Arachidonic Acid
• Carrageenan
• Arachidonate 5-Lipoxygenase
• Cyclooxygenase 1
• Cyclooxygenase 2

Topics

• Amino Acid Sequence
• Animals
• Anti-Inflammatory Agents (chemistry)
• Arachidonate 5-Lipoxygenase (metabolism)
• Arachidonic Acid (chemistry)
• Arthritis (physiopathology)
• Carrageenan (chemistry)
• Cyclooxygenase 1 (metabolism)
• Cyclooxygenase 2 (metabolism)
• Cyclooxygenase Inhibitors (chemistry)
• Edema
• Leukotrienes (chemistry)
• Lipoxygenase Inhibitors (chemistry)
• Molecular Docking Simulation
• Molecular Sequence Data
• Prostaglandins (chemistry)
• Protein Conformation
• Rats
• Rats, Wistar
• alpha-Linolenic Acid (chemistry)