EIF5A2 is a novel chemoresistance gene in breast cancer.

Abstract

BACKGROUND:

The eIF5A2 gene (encoding the eukaryotic initiation factor 5A2) located at 3q26 is a putative oncogene that is overexpressed in colon and rectal carcinomas, lung cancer and hepatocellular carcinoma. EIF5A2 overexpression correlates significantly with tumor metastasis and is an adverse prognostic marker. However, eIF-5A2 overexpression in breast cancer and its effect on chemotherapy are unknown.

METHODS:

We measured eIF-5A2 expression and doxorubicin sensitivity in different human breast cancer cell lines (Bcap-1937, HCC1937, and MCF-7). To investigate a role for eIF-5A2 in chemoresistance, cells were treated with eIF-5A2-siRNA, exposed to various concentrations of doxorubicin, and toxicity was assayed by CCK-8 (cell counting kit).

RESULTS:

The eIF-5A2 expression levels varied among breast cancer cells. Higher expression levels correlated with decreased doxorubicin sensitivity. Silencing of eIF-5A2 significantly improved doxorubicin toxicity in all three breast cancer cell lines.

CONCLUSION:

This study shows that eIF-5A2 plays an important role in doxorubicin chemoresistance in breast cancer cells.

• Authors: Yu Liu, Feiya Du, Wei Chen, Minya Yao, Kezhen Lv, Peifen Fu
• Journal: Breast cancer (Tokyo, Japan) (Breast Cancer) Vol. 22 Issue 6 Pg. 602-7 (Nov 2015) ISSN: 1880-4233 [Electronic] Japan
• PMID: 24638963 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibiotics, Antineoplastic
• Peptide Initiation Factors
• RNA, Small Interfering
• RNA-Binding Proteins
• eukaryotic translation initiation factor 5A
• Doxorubicin

Topics

• Antibiotics, Antineoplastic (pharmacology)
• Breast Neoplasms (drug therapy, genetics, pathology)
• Cell Line, Tumor (drug effects)
• Doxorubicin (pharmacology)
• Drug Resistance, Neoplasm (genetics)
• Female
• Gene Expression Regulation, Neoplastic (drug effects)
• Gene Knockdown Techniques
• Humans
• MCF-7 Cells (drug effects)
• Peptide Initiation Factors (genetics, metabolism)
• RNA, Small Interfering
• RNA-Binding Proteins (genetics, metabolism)