Overexpression of a stress-responsive MYB transcription factor of Poncirus trifoliata confers enhanced dehydration tolerance and increases polyamine biosynthesis.

Abstract

MYBs are an important family of transcription factors that play significant roles in plant development and stress response in plants. However, knowledge concerning the functions of MYBs in the non-model plants and the target genes is still limited. In this study, we isolated a stress-responsive R2R3-type MYB gene from trifoliate orange (Poncirus trifoliata (L.) Raf.), designated as PtsrMYB. PtsrMYB shares the highest degree of identity with AtMYB109. Subcellular localization using onion epidermal cells indicates that PtsrMYB is localized in the nucleus. Transcript levels of PtsrMYB were up-regulated by abiotic stresses such as dehydration, salt, cold and ABA treatment. Overexpression of PtsrMYB in tobacco confers enhanced dehydration tolerance, as indicated by less water loss, lower levels of malondialdehyde and reactive oxygen species. The transgenic tobacco lines displayed higher mRNA levels of two arginine decarboxylase (ADC) genes before and after dehydration treatment when compared with the wild type, concurrent with the greater levels of polyamines. Several MYB-recognizing cis-acting elements exist on the promoters of PtADC gene. Yeast one-hybrid assay demonstrated that PtsrMYB predominantly interact with two regions of the promoter, indicating the PtADC may be a target gene of PtsrMYB. Take together, PtsrMYB plays a positive role in dehydration tolerance, which may be, at least in part, due to the modulation of polyamine synthesis by regulating the ADC gene.

• Authors: Peipei Sun, Xiaofang Zhu, Xiaosan Huang, Ji-Hong Liu
• Journal: Plant physiology and biochemistry : PPB (Plant Physiol Biochem) Vol. 78 Pg. 71-9 (May 2014) ISSN: 1873-2690 [Electronic] France
• PMID: 24636909 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Masson SAS. All rights reserved.

Chemical References

• Plant Growth Regulators
• Plant Proteins
• Polyamines
• Reactive Oxygen Species
• Transcription Factors
• Sodium Chloride
• Malondialdehyde
• Abscisic Acid
• Carboxy-Lyases
• arginine decarboxylase

Topics

• Abscisic Acid (pharmacology)
• Adaptation, Physiological (genetics)
• Amino Acid Sequence
• Carboxy-Lyases (genetics, metabolism)
• Cell Nucleus (metabolism)
• Cold Temperature
• Dehydration
• Gene Expression Regulation, Plant (drug effects)
• Malondialdehyde (metabolism)
• Microscopy, Fluorescence
• Molecular Sequence Data
• Onions (cytology, genetics, metabolism)
• Plant Epidermis (cytology, metabolism)
• Plant Growth Regulators (pharmacology)
• Plant Proteins (genetics, metabolism)
• Plants, Genetically Modified
• Polyamines (metabolism)
• Poncirus (genetics, metabolism)
• Reactive Oxygen Species (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Sequence Homology, Amino Acid
• Sodium Chloride (pharmacology)
• Tobacco (genetics, metabolism)
• Transcription Factors (genetics, metabolism)