Herbal drug ninjin'yoeito accelerates myelopoiesis but not erythropoiesis in vitro.

Abstract

Some Kampo medicines that are herbal and traditional in Japan have had beneficial effects when given to patients with anemia. However, molecular mechanisms underlying their effects are unclear. To address this question, four Kampo medicines used to treat anemia-ninjin'yoeito (NYT), shimotsuto (SMT), juzentaihoto (JTT), and daibofuto (DBT)-were tested separately using in vitro cultures of mouse bone marrow mononuclear cells. Among them, NYT was most effective in stimulating cell proliferation and up-regulating Myc expression. Flow cytometry analysis indicated that, among hematopoietic components of those cultures, myeloid cells expressing CD45/Mac-1/Gr-1/F4/80 increased in number, but Ter119/CD71 erythroid cells did not. Accordingly, real-time PCR analysis showed up-regulation of the myeloid gene Pu.1, whereas the erythroid genes Gata1 and Klf1 were down-regulated. Overall, these findings provide molecular evidence that NYT accelerates myelopoiesis but not erythropoiesis in vitro.

• Authors: Tomoko Inoue, Kasem Kulkeaw, Kanitta Muennu, Yuka Tanaka, Yoichi Nakanishi, Daisuke Sugiyama
• Journal: Genes to cells : devoted to molecular & cellular mechanisms (Genes Cells) Vol. 19 Issue 5 Pg. 432-40 (May 2014) ISSN: 1365-2443 [Electronic] England
• PMID: 24636045 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 The Authors Genes to Cells © 2014 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.

Chemical References

• GATA1 Transcription Factor
• Gata1 protein, mouse
• Kruppel-Like Transcription Factors
• Plant Extracts
• Proto-Oncogene Proteins
• Trans-Activators
• erythroid Kruppel-like factor
• proto-oncogene protein Spi-1

Topics

• Anemia (drug therapy)
• Animals
• Bone Marrow Cells (cytology, drug effects)
• Cell Proliferation
• Cells, Cultured
• Erythropoiesis (drug effects)
• GATA1 Transcription Factor (genetics, metabolism)
• Genes, myc
• Kruppel-Like Transcription Factors (genetics, metabolism)
• Mice
• Mice, Inbred C57BL
• Myelopoiesis (drug effects)
• Panax (chemistry)
• Plant Extracts (pharmacology)
• Proto-Oncogene Proteins (genetics, metabolism)
• Trans-Activators (genetics, metabolism)