Abstract | UNLABELLED:
Estrogen (E(2)) and progesterone (P) hormones have a pro-inflammatory and an anti-inflammatory role under different conditions. The current study explored this phenomenon in the context of septic inflammation. MATERIALS AND METHODS: This study involved 48 female albino rats. E(2) (4 mg/100 g body weight (b.w.) and P (5 mg/kg b.w.) were administered to ovariectomized (OVX) rats after systemic inflammation (SI) induced by puncturing the caecum I cm from its end with a single hole by using a 21-gauge needle. Key indices of inflammation and apoptosis were evaluated. RESULTS: OVX animals subjected to SI showed significantly increased levels of serum tumor necrosis factor-alpha (TNF-u), C reactive protein (CRP) and alanine aminotransferase (ALT). They also showed higher levels of expression of the enzyme inducible nitric oxide synthase (iN OS); 312 ± 43 mg/ml; in the liver, and the activity of both cyclooxygenase 2 (COX-2); 59.4 ± 3.2 U/ml; and caspase 3 enzymes; 6.3 ± 0.54 ng/ml; when compared to non-OVX animals subjected to (SI), (180 ± 3 mg/ml, 16.4 ± 1.69 U/ml, 0.98 ± 0.23 ng/ml respectively). Administration of E(2) resulted in a significant reduction of all serum and liver tissue parameters of inflammation (e.g.decreased iNOS; 193 ± 28 mg/ml and COX-2; 27.6 ± 3.91 U/ml) and decreased apoptosis ( Caspase 3; 1.18 ± 0.21 ng/ml). In contrast, OVX animals injected with P before induction of SI showed a significant rise of all measured parameters. CONCLUSIONS: E(2) and Pin physiological levels have contrasting though complementary roles in regulation of the immune system possibly allowing a limited inflammatory response while preventing excessive damage to the tissues.
|
Authors | A Hassouna, E Obaia, S Marzouk, M Rateb, Mohamed Haidara |
Journal | Acta physiologica Hungarica
(Acta Physiol Hung)
Vol. 101
Issue 1
Pg. 112-27
(Mar 2014)
ISSN: 0231-424X [Print] Hungary |
PMID | 24631798
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Anti-Inflammatory Agents
- Carrier Proteins
- Crp protein, rat
- Inflammation Mediators
- Tumor Necrosis Factor-alpha
- Progesterone
- Estradiol
- Nitric Oxide Synthase Type II
- Nos2 protein, rat
- Cyclooxygenase 2
- Ptgs2 protein, rat
- Alanine Transaminase
- Casp3 protein, rat
- Caspase 3
|
Topics |
- Alanine Transaminase
(blood)
- Animals
- Anti-Inflammatory Agents
(administration & dosage)
- Apoptosis
- Carrier Proteins
(blood)
- Caspase 3
(metabolism)
- Cecum
(microbiology, surgery)
- Cyclooxygenase 2
(metabolism)
- Disease Models, Animal
- Estradiol
(administration & dosage, blood, metabolism)
- Estrogen Replacement Therapy
- Female
- Inflammation
(metabolism, microbiology, pathology, prevention & control)
- Inflammation Mediators
(blood, metabolism)
- Liver
(metabolism, pathology)
- Nitric Oxide Synthase Type II
(metabolism)
- Ovariectomy
- Progesterone
(administration & dosage, blood, metabolism)
- Punctures
- Rats
- Tumor Necrosis Factor-alpha
(blood)
|