Abstract |
This study aimed to determine the in vitro effect of cadmium on the differentiation of duck embryonic bone marrow cells into osteoclasts. Bone marrow cells were harvested from 23-day old Gaoyou duck embryos and were cultured with either 50 nmol/L cadmium alone or different cadmium concentrations (0, 5, 10, 20 and 50 nmol/L) in combination with macrophage colony stimulating factor ( M-CSF) and receptor activator of nuclear factor κB ligand (RANKL). Tartrate-resistant acid phosphatase (TRAP) staining, pit formation assay with bovine cortical bone slices, and co-staining with tetramethyl rhodamine isothiocyanate ( TRITC)-conjugated phalloidin and Hoechst 33258 were performed to determine the number of TRAP-positive cells and bone resorption activity. Cadmium at a concentration ⩾ 10 nmol/L in the presence of M-CSF and RANKL significantly increased in a concentration-dependent manner both the number of TRAP-positive cells (35-160%) and bone resorption activity (36-261%) (P<0.05). High cadmium concentrations in the presence of M-CSF and RANKL markedly promoted the formation of filamentous ( F)-actin rings in differentiated osteoclasts. In conclusion, cadmium promotes in vitro the differentiation of duck embryonic osteoclasts in the presence of M-CSF and RANKL.
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Authors | Yi Wang, Ying-Xiao Fu, Jian-Hong Gu, Yan Yuan, Xue-Zhong Liu, Jian-Chun Bian, Zong-Ping Liu |
Journal | Veterinary journal (London, England : 1997)
(Vet J)
Vol. 200
Issue 1
Pg. 181-5
(Apr 2014)
ISSN: 1532-2971 [Electronic] England |
PMID | 24630041
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Ltd. All rights reserved. |
Chemical References |
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Topics |
- Animals
- Bone Marrow Cells
(cytology, drug effects, metabolism)
- Cadmium
(toxicity)
- Cell Differentiation
(drug effects)
- Cells, Cultured
- Ducks
(embryology)
- Embryo, Nonmammalian
(cytology, drug effects, metabolism)
- Osteoclasts
(cytology, drug effects)
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