Interleukin 20 protein locates to distinct mononuclear cells in psoriatic skin.

Abstract

We previously demonstrated that mRNA for the pro-inflammatory cytokine interleukin 20 (IL-20) is expressed in suprapapillary keratinocytes of lesional psoriatic skin (LS). Here, we describe the distribution of IL-20 protein and the identity of the IL-20-positive cells in LS. We found that the main part of IL-20 immunoreactivity is present in mononuclear cells of the dermal papillae, and that the IL-20-positive cells located in the papillae were langerin+, CD1a+, CD4+ and CD303+. These cells might be immature dendritic cell. In situ hybridization for IL-20 mRNA on non-LS, ex vivo stimulated with IL-1β revealed a colocalization between IL-20 mRNA and the keratinocyte marker CK14. No IL-20 mRNA was detected in the dermal mononuclear cells. Our results suggest that IL-20 is produced by keratinocytes, released into the epidermis and then possibly taken up by papillary mononuclear cells. Our study supports that IL-20 is involved in the pathogenesis of psoriasis.

• Authors: Rikke Bech, Kristian Otkjaer, Svend Birkelund, Thomas Vorup-Jensen, Ralf Agger, Claus Johansen, Lars Iversen, Knud Kragballe, John Rømer
• Journal: Experimental dermatology (Exp Dermatol) Vol. 23 Issue 5 Pg. 349-52 (May 2014) ISSN: 1600-0625 [Electronic] Denmark
• PMID: 24628979 (Publication Type: Letter, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Antigens, CD
• Antigens, CD1
• CD1a antigen
• CD207 protein, human
• CD4 Antigens
• CLEC4C protein, human
• Interleukin-1beta
• Interleukins
• Keratin-14
• Lectins, C-Type
• Mannose-Binding Lectins
• Membrane Glycoproteins
• Receptors, Immunologic
• interleukin 20

Topics

• Antigens, CD (metabolism)
• Antigens, CD1 (metabolism)
• CD4 Antigens (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Epidermis (metabolism)
• HEK293 Cells
• Humans
• Interleukin-1beta (metabolism)
• Interleukins (metabolism)
• Keratin-14 (metabolism)
• Keratinocytes (metabolism)
• Lectins, C-Type (metabolism)
• Leukocytes, Mononuclear (metabolism)
• Mannose-Binding Lectins (metabolism)
• Membrane Glycoproteins (metabolism)
• Psoriasis (metabolism)
• Receptors, Immunologic (metabolism)
• Skin (metabolism)