NRP-1 expression in bladder cancer and its implications for tumor progression.

Abstract

Neuropilin-1 (NRP-1) overexpression has been reported in a variety of human cancers. However, the role of NRP-1 in bladder cancer (BC) remains unclear. The aim of present study was to analyze NRP-1 protein expression in BC tissues and to assess its prognostic significance for BC. NRP-1 messenger ribonucleic acid (mRNA) and protein expression were determined by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and immunohistochemistry in specimens of primary cancer and their adjacent noncancerous tissues in BC patients. Additionally, NRP-1 protein expression in 139 archived paraffin-embedded BC samples was analyzed by immunohistochemistry and correlated with clinicopathological characteristics and survival. Student's t test, Spearman's rank correlation, Kaplan-Meier plots, and Cox's proportional hazards regression model were used to analyze the data. By qRT-PCR and immunohistochemistry, the levels of NRP-1 mRNA and protein were significantly higher in BC, compared to that in adjacent noncancerous tissues (P<0.001). High expression of NRP-1 was significantly associated with histologic grade (P=0.016) and tumor stage (P=0.001). Multivariate analysis showed that high expression of NRP-1 was an independent prognostic factor for overall survival. Our study suggests that overexpression of NRP-1 may play an important role in the progression of BC, and NRP-1 expression may serve as a biomarker for poor prognosis for BC.

• Authors: Wen Cheng, Dian Fu, Zhi-Feng Wei, Feng Xu, Xiao-Feng Xu, You-Huang Liu, Jing-Ping Ge, Feng Tian, Cong-Hui Han, Zheng-Yu Zhang, Li-Ming Zhou
• Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 35 Issue 6 Pg. 6089-94 (Jun 2014) ISSN: 1423-0380 [Electronic] Netherlands
• PMID: 24627131 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Neuropilin-1

Topics

• Adult
• Aged
• Biomarkers, Tumor
• Disease Progression
• Female
• Humans
• Immunohistochemistry
• Male
• Middle Aged
• Neuropilin-1 (analysis, genetics, physiology)
• Proportional Hazards Models
• Urinary Bladder Neoplasms (etiology, mortality, pathology)