Abstract |
The modes of genotoxicity of a novel macromolecular antitumor antibiotic (SN-07) were examined using both prokaryotic and eukaryotic cells in vitro. The antibiotic induced a frameshift-type reverse mutation in Ames Salmonella typhimurium TA98 at 1.6-400 ng/plate with and without S9 mix. SN-07 also induced chromosomal aberrations and a forward mutation (6-TGr) in Chinese hamster V79 cells after 1 h treatment at 12.5-100 ng/ml without metabolic activation. The alkaline elution technique revealed that SN-07 induced interstrand DNA cross-linking dose-dependently after treatment with 2.5-10 micrograms/ml for 1 h followed by elution at pH 12.1, but it did not induce the dose-dependent cross-linking after the same treatment followed by elution at pH 12.6. It was also found that SN-07 induced single-strand DNA breaks (pH 12.1) and alkali-labile (pH 12.6) sites after treatment with 0.1-10 micrograms/ml for 1 h followed by 24-h post-incubation.
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Authors | N Yajima, S Ishida, N Miyata, T Kishi, G Kawanishi |
Journal | Mutation research
(Mutat Res)
Vol. 210
Issue 1
Pg. 165-72
(Jan 1989)
ISSN: 0027-5107 [Print] Netherlands |
PMID | 2462669
(Publication Type: Journal Article)
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Chemical References |
- Anthracyclines
- Antibiotics, Antineoplastic
- Cross-Linking Reagents
- SN-07
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Topics |
- Animals
- Anthracyclines
- Antibiotics, Antineoplastic
(toxicity)
- Cells, Cultured
- Chromosome Aberrations
- Cricetinae
- Cross-Linking Reagents
- DNA Damage
- Mutagenicity Tests
- Salmonella typhimurium
(drug effects)
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