Effects of 8-methoxypsoralen and ultraviolet light A on EGF receptor (HER-1) expression.

Abstract

Activation of psoralens by ultraviolet light irradiation at 308-400 nm (UVA) is used in the photochemical treatment of psoriasis. While the major effect of this activation is the formation of DNA adducts, it was recently demonstrated that psoralens can also bind to specific saturable high affinity cellular receptors, and that this is associated with inhibition of epidermal growth factor (EGF)-receptor binding. In view of these findings, we have examined whether 8-methoxy-psoralen (8-MOP) itself, or in combination with UVA, influences expression of the human EGF-receptor gene ("HER-1") in a human keratinocyte cell line. We have found that 8 MOP alone, and to a lesser extent UVA, induce a striking increase in cellular levels of HER-1 RNA. The combination of 8-MOP with UVA produces less induction of HER-1 RNA than that obtained with 8-MOP alone. We suggest, therefore, that this effect of 8-MOP is not due to DNA damage, but may reflect a separate effect of this compound on receptor-mediated signal transduction.

• Authors: X Y Yang, Z A Ronai, R M Santella, I B Weinstein
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 157 Issue 2 Pg. 590-6 (Dec 15 1988) ISSN: 0006-291X [Print] United States
• PMID: 2462418 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• RNA, Messenger
• Keratins
• ErbB Receptors
• Methoxsalen

Topics

• Blotting, Northern
• Dose-Response Relationship, Drug
• Epidermis (physiology)
• ErbB Receptors (genetics)
• Gene Expression Regulation (drug effects, radiation effects)
• Humans
• In Vitro Techniques
• Keratins
• Methoxsalen (pharmacology)
• PUVA Therapy
• RNA, Messenger (genetics)
• Time Factors
• Ultraviolet Rays