HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Diagnostic efficacy of a recombinant cysteine protease of Spirometra erinacei larvae for serodiagnosis of sparganosis.

Abstract
The mature domain of a cysteine protease of Spirometra erinacei plerocercoid larva (i.e., sparganum) was expressed in Escherichia coli, and its value as an antigen for the serodiagnosis of sparganosis was investigated. The recombinant protein (rSepCp-1) has the molecular weight of 23.4 kDa, and strongly reacted with the sparganum positive human or mice sera but not with negative sera by immunoblotting. ELISA with rSepCp-1 protein or sparganum crude antigen (SeC) was evaluated for the serodiagnosis of sparganosis using patient's sera. The sensitivity and specificity of ELISA using rSepCp-1 protein were 95.0% (19/20) and 99.1% (111/112), respectively. In contrast, the sensitivity and specificity of ELISA with SeC were 100% (20/20) and 96.4% (108/112), respectively. Moreover, in experimentally infected mice, the sensitivity and specificity of both ELISA assays were 100% for the detection of anti-sparganum IgG. It is suggested that the rSepCp-1 protein-based ELISA could provide a highly sensitive and specific assay for the diagnosis of sparganosis.
AuthorsS M Mazidur Rahman, Jae-Hwan Kim, Sung-Tae Hong, Min-Ho Choi
JournalThe Korean journal of parasitology (Korean J Parasitol) Vol. 52 Issue 1 Pg. 41-6 (Feb 2014) ISSN: 1738-0006 [Electronic] Korea (South)
PMID24623880 (Publication Type: Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Helminth
  • Recombinant Proteins
  • Cysteine Proteases
Topics
  • Animals
  • Antigens, Helminth (chemistry, genetics)
  • Cloning, Molecular
  • Cysteine Proteases (chemistry, genetics)
  • Enzyme-Linked Immunosorbent Assay
  • Escherichia coli (genetics)
  • Gene Expression
  • Humans
  • Mice
  • Molecular Weight
  • Parasitology (methods)
  • Recombinant Proteins (chemistry, genetics)
  • Sensitivity and Specificity
  • Serologic Tests (methods)
  • Sparganosis (diagnosis)
  • Spirometra (enzymology, genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: