Nanoparticles of star-like copolymer mannitol-functionalized poly(lactide)-vitamin E TPGS for delivery of paclitaxel to prostate cancer cells.

Abstract

The purpose of this research was to develop novel nanoparticles (NPs) of star-like copolymer mannitol-functionalized poly(lactide)-vitamin E TPGS (M-PLA-TPGS) for paclitaxel delivery for prostate cancer treatment, and evaluate their therapeutic effects in prostate cancer cell line and animal model in comparison with the linear PLGA NPs and poly(lactide)-vitamin E TPGS (PLA-TPGS) NPs. The paclitaxel-loaded M-PLA-TPGS NPs, prepared by a modified nano-precipitation method, were observed by FESEM to be near-spherical shape with narrow size distribution. The drug-loaded NPs were further characterized in terms of size, surface charge, drug content, encapsulation efficiency and in vitro drug release. The results showed that the M-PLA-TPGS NPs were found to be stable, showing almost no change in particle size and surface charge during the three-month storage period. In vitro drug release exhibited biphasic pattern with initial burst release followed by slow and continuous release. The cellular uptake level of M-PLA-TPGS NPs was demonstrated higher than linear PLGA NPs and PLA-TPGS NPs in PC-3 cells. The data also showed that the paclitaxel-loaded M-PLA-TPGS nanoparticles have higher antitumor efficacy than that of linear PLA-TPGS nanoparticles and PLGA nanoparticles in vitro and in vivo. In summary, the star-like copolymer M-PLA-TPGS could be used as a potential and promising molecular biomaterial in developing novel nanoformulation for prostate cancer treatment.

• Authors: Kebing Wang, Longhua Guo, Wei Xiong, Leilei Sun, Yi Zheng
• Journal: Journal of biomaterials applications (J Biomater Appl) Vol. 29 Issue 3 Pg. 329-40 (Sep 2014) ISSN: 1530-8022 [Electronic] England
• PMID: 24621530 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

Chemical References

• Antineoplastic Agents, Phytogenic
• Polyesters
• Vitamin E
• poly(lactide)
• Paclitaxel

Topics

• Antineoplastic Agents, Phytogenic (administration & dosage, therapeutic use)
• Cell Line, Tumor
• Humans
• Male
• Nanoparticles
• Paclitaxel (administration & dosage, therapeutic use)
• Polyesters (administration & dosage)
• Prostatic Neoplasms (drug therapy, pathology)
• Proton Magnetic Resonance Spectroscopy
• Vitamin E (administration & dosage)