Polymorphisms in DNA repair genes (APEX1, XPD, XRCC1 and XRCC3) and risk of preeclampsia in a Mexican mestizo population.

Abstract

Variations in genes involved in DNA repair systems have been proposed as risk factors for the development of preeclampsia (PE). We conducted a case-control study to investigate the association of Human apurinic/apyrimidinic (AP) endonuclease (APEX1) Asp148Glu (rs1130409), Xeroderma Pigmentosum group D (XPD) Lys751Gln (rs13181), X-ray repair cross-complementing group 1 (XRCC) Arg399Gln (rs25487) and X-ray repair cross-complementing group 3 (XRCC3) Thr241Met (rs861539) polymorphisms with PE in a Mexican population. Samples of 202 cases and 350 controls were genotyped using RTPCR. Association analyses based on a χ2 test and binary logistic regression were performed to determine the odds ratio (OR) and a 95% confidence interval (95% CI) for each polymorphism. The allelic frequencies of APEX1 Asp148Glu polymorphism showed statistical significant differences between preeclamptic and normal women (p = 0.036). Although neither of the polymorphisms proved to be a risk factor for the disease, the APEX1 Asp148Glu polymorphism showed a tendency of association (OR: 1.74, 95% CI = 0.96-3.14) and a significant trend (p for trend = 0.048). A subgroup analyses revealed differences in the allelic frequencies of APEX1 Asp148Glu polymorphism between women with mild preeclampsia and severe preeclampsia (p = 0.035). In conclusion, our results reveal no association between XPD Lys751Gln, XRCC Arg399Gln and XRCC3 Thr241Met polymorphisms and the risk of PE in a Mexican mestizo population; however, the results in the APEX1 Asp148Glu polymorphism suggest the need for future studies using a larger sample size.

• Authors: Ada Sandoval-Carrillo, Edna M Méndez-Hernández, Fernando Vazquez-Alaniz, Marisela Aguilar-Durán, Alfredo Téllez-Valencia, Marcelo Barraza-Salas, Francisco X Castellanos-Juárez, Osmel La Llave-León, José M Salas-Pacheco
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 15 Issue 3 Pg. 4273-83 (Mar 11 2014) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 24619222 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA-Binding Proteins
• X-ray Repair Cross Complementing Protein 1
• X-ray repair cross complementing protein 3
• XRCC1 protein, human
• Xeroderma Pigmentosum Group D Protein
• APEX1 protein, human
• DNA-(Apurinic or Apyrimidinic Site) Lyase
• ERCC2 protein, human

Topics

• Adolescent
• Adult
• Case-Control Studies
• Chi-Square Distribution
• DNA Repair (genetics)
• DNA-(Apurinic or Apyrimidinic Site) Lyase (genetics)
• DNA-Binding Proteins (genetics)
• Female
• Gene Frequency
• Genetic Predisposition to Disease (genetics)
• Genotype
• Humans
• Logistic Models
• Mexico
• Odds Ratio
• Polymorphism, Single Nucleotide
• Pre-Eclampsia (genetics, pathology)
• Pregnancy
• Risk Factors
• Severity of Illness Index
• X-ray Repair Cross Complementing Protein 1
• Xeroderma Pigmentosum Group D Protein (genetics)
• Young Adult