Clinical significance of polypeptide N-acetylgalactosaminyl transferase-5 (GalNAc-T5) expression in patients with gastric cancer.

Abstract

BACKGROUND:

The family of polypeptide N-acetylgalactosaminyl transferases is responsible for the altered O-linked glycosylation occurring during the development of various cancers and their progression via altering O-glycan biosynthesis. Our studies were designed to investigate the expression and prognostic values of GalNAc-T5 and improve the risk stratification in patients with gastric cancer.

METHODS:

Tissue samples from a training set and a validation set of patients with gastric adenocarcinoma from China were used for analyses. GalNAc-T5 expression was retrospectively analysed by immunohistochemistry. Results were assessed for association with clinicopathological parameters and overall survival by using Kaplan-Meier analysis. Prognostic values of GalNAc-T5 expression and clinical outcomes were evaluated by Cox regression analysis. A molecular prognostic stratification scheme incorporating GalNAc-T5 expression was determined by using receiver operating characteristic analysis.

RESULTS:

GalNAc-T5 expression was markedly reduced in gastric cancer tissues compared with non-malignant gastric mucosa. Low intratumoral GalNAc-T5 density, which was associated with tumour cell differentiation, T classification, N classification, and TNM stage in the two independent sets, was an independent prognosticator for poor prognosis of gastric cancer patients. Applying the prognostic value of intratumoral GalNAc-T5 density to the conventional clinicopathologic TNM stage system showed a better prognostic value in patients with gastric cancer.

CONCLUSIONS:

Intratumoral GalNAc-T5 expression was recognised as an independent prognostic marker for the overall survival of gastric cancer patients. Detection of GalNAc-T5 expression in gastric cancer tissues might add some prognostic information for patients with this disease and lead to a more accurate classification under the TNM stage system.

• Authors: H He, Z Shen, H Zhang, X Wang, Z Tang, J Xu, Y Sun
• Journal: British journal of cancer (Br J Cancer) Vol. 110 Issue 8 Pg. 2021-9 (Apr 15 2014) ISSN: 1532-1827 [Electronic] England
• PMID: 24619076 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• N-Acetylgalactosaminyltransferases
• polypeptide N-acetylgalactosaminyltransferase

Topics

• Aged
• Biomarkers, Tumor (genetics)
• Disease-Free Survival
• Female
• Gene Expression Regulation, Neoplastic
• Glycosylation
• Humans
• Kaplan-Meier Estimate
• Male
• Middle Aged
• Molecular Targeted Therapy
• N-Acetylgalactosaminyltransferases (genetics)
• Neoplasm Staging
• Prognosis
• Stomach Neoplasms (drug therapy, genetics, pathology)