Human autoantibody secreted by immortalized lymphocyte cell line against the 68K polypeptide of the U1 small nuclear ribonucleoprotein.

Abstract

Autoantibody of an immortalized human lymphocyte cell line, Su-2E4, derived from peripheral lymphocytes of a patient with mixed connective tissue disease, showed specific binding of the 68K polypeptide of U1 small nuclear RNP (snRNP) and immunoprecipitation of U1 RNA. The reaction patterns of Su-2E4 and a murine monoclonal anti-(U1)snRNP line, 2.73, and results of a competition assay with the 2 antibodies suggest similar, but not necessarily identical, epitope recognition.

• Authors: J T Chen, Y Takeda, W E Vanderslice, G C Sharp, I Pettersson, A Rosén, H Wigzell, R J Wang
• Journal: Arthritis and rheumatism (Arthritis Rheum) Vol. 31 Issue 10 Pg. 1265-71 (Oct 1988) ISSN: 0004-3591 [Print] United States
• PMID: 2460104 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Autoantibodies
• Peptides
• Ribonucleoproteins
• Ribonucleoproteins, Small Nuclear
• RNA

Topics

• Autoantibodies (analysis, immunology)
• Binding, Competitive
• Cell Line
• Enzyme-Linked Immunosorbent Assay
• Humans
• Immunoblotting
• Lymphocytes (immunology)
• Mixed Connective Tissue Disease (immunology)
• Molecular Weight
• Peptides (immunology)
• Precipitin Tests
• RNA (analysis)
• Ribonucleoproteins (genetics, immunology)
• Ribonucleoproteins, Small Nuclear