Abstract |
The major mature env-gene products of avian reticuloendotheliosis-associated virus (REV-A) are the surface glycoprotein (gp90) and the transmembrane protein (gp20). We have previously reported that gp90 was detected in the REV-A virus by Western blot analysis as well as in the REV-A-infected cells by radioimmunoprecipitation with antibodies raised in rabbits against the gp90 C-terminal tridecapeptide which was predicted from the nucleotide sequence (Wilhemsen et al., J. Virol., 52, 172, 1984). We have now shown that this antibody detected antigens on the REV-A-infected cells by fluorescence-activated cell sorter (FACS) analysis, and conferred specific cytotoxic effects on the infected cells in the presence of rabbit complement using the chromium release assay. These results clearly indicate that the C-terminal epitope of gp90 is situated on the surface of the REV-A-infected cells and accessible to site-directed antibodies which cause cytotoxicity by activating the complement system. The possible in vivo roles of this antibody are discussed.
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Authors | W P Tsai, S Oroszlan |
Journal | Virology
(Virology)
Vol. 166
Issue 2
Pg. 608-11
(Oct 1988)
ISSN: 0042-6822 [Print] United States |
PMID | 2459845
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies, Viral
- Antigens, Surface
- Antigens, Viral
- Epitopes
- Oligopeptides
- Viral Envelope Proteins
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Topics |
- Animals
- Antibodies, Viral
(immunology)
- Antigens, Surface
(immunology)
- Antigens, Viral
(immunology)
- Cells, Cultured
- Cytotoxicity, Immunologic
- Epitopes
- Oligopeptides
(immunology)
- Reticuloendotheliosis virus
(immunology)
- Retroviridae
(immunology)
- Viral Envelope Proteins
(immunology)
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