Tissue engineering scaffolds of mesoporous magnesium silicate and poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) composite.

Abstract

Mesoporous magnesium silicate (m-MS) and poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL) composite scaffolds were fabricated by solvent-casting and particulate leaching method. The results suggested that the incorporation of m-MS into PCL-PEG-PCL could significantly improve the water adsorption of the m-MS/PCL-PEG-PCL composite (m-MPC) scaffolds. The in vitro degradation behavior of m-MPC scaffolds were determined by testing weight loss of the scaffolds after soaking into phosphate buffered saline (PBS), and the result showed that the degradation of m-MPC scaffolds was obviously enhanced by addition of m-MS into PCL-PEG-PCL after soaking for 10 weeks. Proliferation of MG63 cells on m-MPC was significantly higher than MPC scaffolds at 4 and 7 days. ALP activity on the m-MPC was obviously higher than MPC scaffolds at 7 days, revealing that m-MPC could promote cell differentiation. Histological evaluation showed that the introduction of m-MS into PCL-PEG-PCL enhanced the efficiency of new bone formation when the m-MPC scaffolds implanted into bone defect of rabbits. The results suggested that the inorganic/organic composite of m-MS and PCL-PEG-PCL scaffolds exhibited good biocompatibility, degradability and osteogenesis.

• Authors: Dawei He, Wei Dong, Songchao Tang, Jie Wei, Zhenghui Liu, Xiaojiang Gu, Ming Li, Han Guo, Yunfei Niu
• Journal: Journal of materials science. Materials in medicine (J Mater Sci Mater Med) Vol. 25 Issue 6 Pg. 1415-24 (Jun 2014) ISSN: 1573-4838 [Electronic] United States
• PMID: 24595904 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bone Substitutes
• Magnesium Silicates
• Polyesters
• poly(epsilon-caprolactone)-b-poly(ethyleneglycol)-b-poly(epsilon-caprolactone)
• Water
• Florisil
• Polyethylene Glycols

Topics

• Absorbable Implants
• Absorption
• Animals
• Bone Substitutes (chemical synthesis)
• Cell Line
• Cell Proliferation
• Crystallization (methods)
• Equipment Design
• Equipment Failure Analysis
• Femoral Fractures (pathology, surgery)
• Humans
• Magnesium Silicates (chemistry)
• Materials Testing
• Osteoblasts (cytology, physiology)
• Osteogenesis
• Polyesters (chemical synthesis)
• Polyethylene Glycols (chemical synthesis)
• Porosity
• Rabbits
• Surface Properties
• Tissue Engineering (instrumentation)
• Tissue Scaffolds
• Treatment Outcome
• Water