Heat shock protein 47 (HSP47), also known as SERPINH1, is a product of
CBP2 gene located at chromosome 11q13.5, a region frequently amplified in human
cancers. HSP47 has been demonstrated to effect on limiting
tumor invasion and motility. The previous studies showed that HSP47 is overexpressed in many human
cancers, including
stomach cancer,
lung cancer, pancreatic ductal
adenocarcinoma, and
ulcerative colitis-associated
carcinomas. However, the role of HSP47 in human
glioma is still unknown. Here, we examined the expression of HSP47 in a group of
glioma tumors and matched non-
tumor brain tissues using qRT-PCR. We found that HSP47 is significantly overexpressed in
glioma tissues and cell lines and associated with
glioma tumor grade. Next, we knockdown the expression of HSP47 in the
glioma cells using small interfering RNAs. The result showed that knockdown of HSP47 inhibits
glioma cell growth, migration and invasion in vitro. We further investigated the posttranscriptional regulation of HSP47 by
microRNAs using bioinformatics analysis and experimental validation. The results suggested that the expression of HSP47 is regulated by miR-29a. Finally, stable knockdown of HSP47 using
shRNA inhibits
glioma tumor growth and induces apoptosis in mice models in vivo. Therefore, our data suggested that HSP47 regulated by miR-29a to enhance
glioma tumor growth and invasion. Taken together, HSP47 plays important role in
tumor growth and invasion and thus could be a therapeutic target for treating
glioma in the future.