Genotoxic evaluation of titanium dioxide nanoparticles in vivo and in vitro.

Abstract

With the extensive application of titanium dioxide (TiO2) nanoparticles (NPs) in food industry, there is a rising debate concerning the possible risk associated with exposure to TiO2 NPs. The purpose of this study is to evaluate the genotoxicity of TiO2 NPs using in vivo and in vitro test systems. In vivo study, the adult male Sprague-Dawley rats were exposed to anatase TiO2 NPs (75 ± 15 nm) through intragastric administration at 0, 10, 50 and 200mg/kg body weight every day for 30 days. The γ-H2AX assay showed TiO2 NPs could induce DNA double strand breaks in bone marrow cells after oral administration. However, the micronucleus test revealed that the oral-exposed TiO2 NPs did not cause damage to chromosomes or mitotic apparatus observably in rat bone marrow cells. In vitro study, Chinese hamster lung fibroblasts (V79 cells) were exposed to TiO2 NPs at the dose of 0, 5, 10, 20, 50 and 100 μg/mL. Significant decreases in cell viability were detected in all the treated groups after 24h and 48h exposure. Significant DNA damage was only observed at the concentration of 100 μg/mL after 24h treatment using the comet assay. The obvious gene mutation was observed at the concentration of 20 and 100 μg/mL after 2h treatment using hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene mutation assay. This study presented a comprehensive genotoxic evaluation of TiO2 NPs, and TiO2 NPs were shown to be genotoxic both in vivo and in vitro tests. The gene mutation and DNA strand breaks seem to be more sensitive genetic endpoints for the detection of TiO2 NPs induced genotoxic effects.

• Authors: Zhangjian Chen, Yun Wang, Te Ba, Yang Li, Ji Pu, Tian Chen, Yanshuang Song, Yongen Gu, Qin Qian, Jinglin Yang, Guang Jia
• Journal: Toxicology letters (Toxicol Lett) Vol. 226 Issue 3 Pg. 314-9 (May 02 2014) ISSN: 1879-3169 [Electronic] Netherlands
• PMID: 24594277 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• titanium dioxide
• Titanium
• Hypoxanthine Phosphoribosyltransferase

Topics

• Animals
• Bone Marrow (drug effects)
• Cells, Cultured
• Cricetinae
• Cricetulus
• DNA Damage
• Dose-Response Relationship, Drug
• Hypoxanthine Phosphoribosyltransferase (genetics)
• Male
• Metal Nanoparticles (toxicity)
• Mutation
• Rats
• Rats, Sprague-Dawley
• Titanium (toxicity)