Immunogenicity and protective efficacy of yeast extracts containing rotavirus-like particles: a potential veterinary vaccine.

Abstract

Rotavirus is the most common cause of severe diarrhea in many animal species of economic interest. A simple, safe and cost-effective vaccine is required for the control and prevention of rotavirus in animals. In this study, we evaluated the use of Saccharomyces cerevisiae extracts containing rotavirus-like particles (RLP) as a vaccine candidate in an adult mice model. Two doses of 1mg of yeast extract containing rotavirus proteins (between 0.3 and 3 μg) resulted in an immunological response capable of reducing the replication of rotavirus after infection. Viral shedding in all mice groups diminished in comparison with the control group when challenged with 100 50% diarrhea doses (DD50) of murine rotavirus strain EDIM. Interestingly, when immunizing intranasally protection against rotavirus infection was observed even when no increase in rotavirus-specific antibody titers was evident, suggesting that cellular responses were responsible of protection. Our results indicate that raw yeast extracts containing rotavirus proteins and RLP are a simple, cost-effective alternative for veterinary vaccines against rotavirus.

• Authors: William A Rodríguez-Limas, Ana Ruth Pastor, Ernesto Esquivel-Soto, Fernando Esquivel-Guadarrama, Octavio T Ramírez, Laura A Palomares
• Journal: Vaccine (Vaccine) Vol. 32 Issue 24 Pg. 2794-8 (May 19 2014) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 24593996 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Chemical References

• Antibodies, Viral
• Antigens, Viral
• Capsid Proteins
• Rotavirus Vaccines
• VP2 protein, Rotavirus
• VP6 protein, Rotavirus
• VP7 protein, Rotavirus
• Vaccines, Virus-Like Particle

Topics

• Animals
• Antibodies, Viral (blood)
• Antibody Formation
• Antigens, Viral (immunology)
• Batch Cell Culture Techniques
• Capsid Proteins (immunology)
• Female
• Mice
• Rotavirus
• Rotavirus Infections (prevention & control)
• Rotavirus Vaccines (immunology)
• Saccharomyces cerevisiae (immunology, virology)
• Vaccines, Virus-Like Particle (immunology)
• Virus Cultivation
• Virus Shedding