Abstract |
NMR has proven to be an invaluable technique for identifying and characterizing ligand interactions with biomolecules. NMR-based detection of ligand binding to protein targets is described. Specifically, the use of the WaterLOGSY NMR experiment to screen mixtures of compounds from a fragment library for binding to influenza H5 hemagglutinin is detailed.
|
Authors | Benjamin E Ramirez, Aleksandar Antanasijevic, Michael Caffrey |
Journal | Methods in molecular biology (Clifton, N.J.)
(Methods Mol Biol)
Vol. 1140
Pg. 305-13
( 2014)
ISSN: 1940-6029 [Electronic] United States |
PMID | 24590726
(Publication Type: Journal Article)
|
Chemical References |
- Hemagglutinin Glycoproteins, Influenza Virus
- Ligands
- hemagglutinin, avian influenza A virus
- Water
|
Topics |
- Drug Evaluation, Preclinical
- Hemagglutinin Glycoproteins, Influenza Virus
(chemistry)
- Humans
- Influenza, Human
(genetics, metabolism)
- Ligands
- Magnetic Resonance Spectroscopy
- Molecular Biology
(methods)
- Protein Binding
- Water
(chemistry)
|