STAT3 is well corroborated preclinically as a
cancer therapeutic target, but tractable translational strategies for its blockade by small molecule inhibitors have remained elusive. In this study, we report the development of a novel class of bifunctional STAT3 inhibitors, based on conjugation of a diarylidenyl-
piperidone (DAP) backbone to an N-hydroxypyrroline (-NOH) group, which exhibits minimal toxicity against normal cells and good oral bioavailability. Molecular modeling studies of this class suggested direct interaction with the STAT3
DNA binding domain. In particular, the DAP compound
HO-3867 selectively inhibited STAT3 phosphorylation, transcription, and
DNA binding without affecting the expression of other active STATs.
HO-3867 exhibited minimal toxicity toward noncancerous cells and tissues but induced apoptosis in
ovarian cancer cells. Pharmacologic analysis revealed greater bioabsorption and bioavailability of the active (cytotoxic) metabolites in
cancer cells compared with normal cells. The selective cytotoxicity of
HO-3867 seemed to be multifaceted, eliciting differential activation of the Akt pathway in normal versus
cancer cells. RNAi attenuation experiments confirmed the requirement of STAT3 for HO-3867-mediated apoptosis in
ovarian cancer cells. In vivo testing showed that
HO-3867 could block xenograft
tumor growth without toxic side effects. Furthermore, in primary human
ovarian cancer cells isolated from patient
ascites,
HO-3867 inhibited cell migration/invasion and survival. Our results offer preclinical proof-of-concept for
HO-3867 as a selective STAT3 inhibitor to treat
ovarian cancer and other solid
tumors where STAT3 is widely upregulated.