Wound healing involves
inflammation followed by granular tissue development and
scar formation. In this study, synthetic
chalcone 3-(2-Chlorophenyl)-1-phenyl-propenone (
CPPP) was investigated for a potential role in enhancing wound healing and closure. Twenty-four male rats were divided randomly into 4 groups:
carboxymethyl cellulose (CMC) (0.2 mL),
Intrasite gel, and
CPPP (25 or 50 mg/mL). Gross morphology,
wounds treatment with the
CPPP, and
Intrasite gel accelerate the rate of wound healing compared to CMC group. Ten days after surgery, the animals were sacrificed. Histological assessment revealed that the
wounds treated with
CPPP showed that
wound closure site contained little amount of
scar and the granulation tissue contained more
collagen and less inflammatory cells than
wound treated with CMC. This finding was confirmed with Masson's trichrome staining. The
antioxidant defence
enzymes catalase (CAT) and
superoxide dismutase (SOD) were significantly increased in the
wound homogenates treated with
CPPP (P < 0.05) compared to CMC treated group. However, in the
CPPP treatment group, lipid peroxidation (MDA) was significantly decreased (P < 0.05), suggesting that the
CPPP also has an important role in protection against lipid peroxidation-induced skin injury after ten days of treatment with
CPPP, which is similar to the values of
cytokines TGF-β and TNF-α in tissue homogenate. Finally the administration of
CPPP at a dosage of 25 and 50 mg/kg was suitable for the stimulation of wound healing.