Abstract | OBJECTIVE: METHODS:
Peritoneal fibrosis was induced by the injection of chlorhexidine gluconate (CG) into the peritoneal cavity of mice every other day for 3 weeks. SAHA, or a dimethylsulfoxide and saline vehicle, was administered subcutaneously every day from the start of the CG injections for 3 weeks. Morphologic peritoneal changes were assessed by Masson's trichrome staining, and fibrosis-associated factors were assessed by immunohistochemistry. RESULTS: In CG-injected mice, a marked thickening of the submesothelial compact zone was observed. In contrast, the administration of SAHA suppressed the progression of submesothelial thickening and type III collagen accumulation in CG-injected mice. The numbers of fibroblast-specific protein-1-positive cells and α-smooth muscle actin α-positive cells were significantly decreased in the CG + SAHA group compared to that of the CG group. The level of histone acetylation was reduced in the peritoneum of the CG group, whereas it was increased in the CG + SAHA group. CONCLUSIONS: Our results indicate that SAHA can suppress peritoneal thickening and fibrosis in mice through up-regulation of histone acetylation. These results suggest that SAHA may have therapeutic potential for treating peritoneal fibrosis.
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Authors | Kumiko Io, Tomoya Nishino, Yoko Obata, Mineaki Kitamura, Takehiko Koji, Shigeru Kohno |
Journal | Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis
(Perit Dial Int)
2015 May-Jun
Vol. 35
Issue 3
Pg. 246-58
ISSN: 1718-4304 [Electronic] United States |
PMID | 24584598
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 International Society for Peritoneal Dialysis. |
Chemical References |
- Fluorine Radioisotopes
- Histone Deacetylase Inhibitors
- Hydroxamic Acids
- Vorinostat
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Topics |
- Animals
- Disease Models, Animal
- Disease Progression
- Fluorine Radioisotopes
- Histone Deacetylase Inhibitors
(administration & dosage)
- Hydroxamic Acids
(administration & dosage)
- Injections, Subcutaneous
- Male
- Mice
- Mice, Inbred ICR
- Peritoneal Dialysis
(adverse effects)
- Peritoneal Fibrosis
(etiology, pathology, prevention & control)
- Vorinostat
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