Severe
acute pancreatitis (SAP) is the sudden onset of pancreatic
inflammation, which is characterized by
edema, acinar cell
necrosis,
hemorrhage and severe
inflammation of the pancreas and is associated with a high mortality rate.
Daphnetin has been shown to alleviate organ injury in a variety of preclinical animal models of coagulation disorders. The aim of the present study was to investigate the protective effects of
daphnetin on severe
acute pancreatitis in a rat model. Severe
acute pancreatitis in the rat model was induced by retrograde infusion of 5%
sodium taurocholate (1 ml/kg) into the bile-pancreatic duct.
Daphnetin (4 mg/kg) was administered intraperitoneally at 30 min prior to the infusion of
sodium taurocholate. The severity of
pancreatitis was evaluated by various analyses of serum
amylase and
lipase,
tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) levels,
myeloperoxidase (MPO) activity and
malondialdehyde (MDA) content, as well as by histological grading. The levels of TNF-α and IL-1β in the serum were measured by ELISA. The results revealed that the
daphnetin-treated SAP rat group (SAP-D) exhibited a lower pathological score of the pancreas compared with the SAP group (SAP). Further analyses demonstrated that the SAP-D group had lower levels of serum
amylase,
lipase and pro-inflammatory
cytokines, including TNF-α and IL-1β, and a decreased MPO activity and MDA content 3, 6 and 12 h subsequent to the infusion of
sodium taurocholate compared with the SAP group (SAP). These findings indicated that
daphnetin exerted a protective function in the SAP rat model. Therefore,
daphnetin may be considered as a potential compound for the
therapy and prevention of
acute pancreatitis.