15-Deoxy-Δ(12,14)-prostaglandin J2 (15d-PGJ2) promotes apoptosis of HBx-positive liver cells.

Abstract

This study aims to investigate the inflammatory response characteristics of liver cells caused by HBV x protein (HBx) and the unique function of the PGE2 inhibitor on HBx-positive liver cells. Tetrazolium blue colorimetric method, flow cytometry, and Western blot were performed to detect the proliferation, cycle, and apoptosis protein expression of HBx-positive HL7702 liver and control cells. The effect of the PGE2 inhibitor 15-Deoxy-Δ(12,14)-prostaglandin J2 (15d-PGJ2) on the growth of HL7702-HBx was also observed. HBx induces the PGE2 accumulation in HL7702 liver cells and promotes their growth and inhibits their apoptosis. HL7702-HBx and HL7702 cells showed increased apoptosis rate, increased apoptosis-promoting protein expression, and reduced apoptosis-inhibiting protein expression under the effect of 15d-PGJ2, and the changes in HL7702-HBx cells were more significant than in HL7702 cells. HBx expression causes liver cells to be more sensitive to the apoptosis-promoting function of 15d-PGJ2. Therefore, the use of 15d-PGJ2 may be a new method for the prevention or treatment of inflammatory changes to cancer caused by HBV infection in liver cells.

• Authors: Siyan Chen, Chong Liu, Xiaoqian Wang, Xiujin Li, Yanling Chen, Nanhong Tang
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 214 Pg. 26-32 (May 05 2014) ISSN: 1872-7786 [Electronic] Ireland
• PMID: 24582817 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• 15-deoxy-delta(12,14)-prostaglandin J2
• DNA Primers
• Trans-Activators
• Viral Regulatory and Accessory Proteins
• hepatitis B virus X protein
• Dinoprostone
• Prostaglandin D2

Topics

• Apoptosis (drug effects)
• Base Sequence
• Blotting, Western
• Cell Line
• Colorimetry
• DNA Primers
• Dinoprostone (antagonists & inhibitors, metabolism)
• Flow Cytometry
• Humans
• Liver (cytology, drug effects)
• Polymerase Chain Reaction
• Prostaglandin D2 (analogs & derivatives, pharmacology)
• Trans-Activators (metabolism)
• Viral Regulatory and Accessory Proteins