Abstract |
This study aims to investigate the inflammatory response characteristics of liver cells caused by HBV x protein (HBx) and the unique function of the PGE2 inhibitor on HBx-positive liver cells. Tetrazolium blue colorimetric method, flow cytometry, and Western blot were performed to detect the proliferation, cycle, and apoptosis protein expression of HBx-positive HL7702 liver and control cells. The effect of the PGE2 inhibitor 15-Deoxy-Δ(12,14)-prostaglandin J2 (15d-PGJ2) on the growth of HL7702-HBx was also observed. HBx induces the PGE2 accumulation in HL7702 liver cells and promotes their growth and inhibits their apoptosis. HL7702-HBx and HL7702 cells showed increased apoptosis rate, increased apoptosis-promoting protein expression, and reduced apoptosis-inhibiting protein expression under the effect of 15d-PGJ2, and the changes in HL7702-HBx cells were more significant than in HL7702 cells. HBx expression causes liver cells to be more sensitive to the apoptosis-promoting function of 15d-PGJ2. Therefore, the use of 15d-PGJ2 may be a new method for the prevention or treatment of inflammatory changes to cancer caused by HBV infection in liver cells.
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Authors | Siyan Chen, Chong Liu, Xiaoqian Wang, Xiujin Li, Yanling Chen, Nanhong Tang |
Journal | Chemico-biological interactions
(Chem Biol Interact)
Vol. 214
Pg. 26-32
(May 05 2014)
ISSN: 1872-7786 [Electronic] Ireland |
PMID | 24582817
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- 15-deoxy-delta(12,14)-prostaglandin J2
- DNA Primers
- Trans-Activators
- Viral Regulatory and Accessory Proteins
- hepatitis B virus X protein
- Dinoprostone
- Prostaglandin D2
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Topics |
- Apoptosis
(drug effects)
- Base Sequence
- Blotting, Western
- Cell Line
- Colorimetry
- DNA Primers
- Dinoprostone
(antagonists & inhibitors, metabolism)
- Flow Cytometry
- Humans
- Liver
(cytology, drug effects)
- Polymerase Chain Reaction
- Prostaglandin D2
(analogs & derivatives, pharmacology)
- Trans-Activators
(metabolism)
- Viral Regulatory and Accessory Proteins
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