[Dendritic projections from meridian-related motoneurons to sympathic preganglion neurons in the spinal cord of rats].

Abstract

OBJECTIVE:

Previous studies indicated a close involvement of reflex activities of motoneurons in the spinal cord in the mechanism of meridian phenomena. The present study was designed to investigate the dendrite projections of meridian-related motoneurons among the motoneurons and sympathetic preganglionic neurons in the spinal cord.

METHODS:

A total of 41 Sprague-Dawley rats were used in the present study. Cholera toxin B-subunit conjugated horseradish peroxidase (CB-HRP) containing 1.0% HRP was respectively injected to acupoint "Chengman" (ST 20), "Liangmen" (ST 21), "Guanmen" (ST 22), "Taiyi" (ST 23), "Huaroumen" (ST 24), "Tianshu" (ST 25) and "Wailing" (ST 26) of the Stomach Meridian, and "Ganshu" (BL 18), "Danshu" (BL 19), "Pishu" (BL 20), "Weishu" (BL 21) and "Sanjiaoshu" (BL 22) of the Bladder Meridian, and "Daimai" (GB 26), "Wushu" (GB 27), "Weidao" (GB 28), "Juliao" (GB 29), "Huantiao" (GB 30), "Fengshi" (GB 31), "Zhongdu" (GB 32), "Xiyangguan" (GB 33) and "Yanglingquan" (GB 34) of the Gallbladder Meridian (for labeling preganglionic neurons), and the celiac ganglion and superior mesenteric ganglion for labeling sympathetic preganglionic neurons. Three days after injection, the animals anesthetized were transcardia-cally perfused with 1.5% paraformaldehyde, the spinal cord was removed to be fixed routinely and then cut into sections for observing the labeled cells under microscope.

RESULTS:

In the ipsilateral ventral horn of the spinal cord, the motoneurons retrogradely labeled by CB-HRP formed dendritic projections oriented only to those motoneurons innervating the same meridian. In the longitudinal sections of spinal cord, the labeled motoneurons formed a bead-like column with a prominent network of longitudinal dendrites connecting the motoneurons innervating acupoints from the same meridian. In the transverse sections of spinal cord, two groups of dendrites from the labeled motoneurons projected to the identified sympathetic preganglionic regions: one group extended dorsolateraly to the intermediolateral gray, another group extended intermediolateraly toward the central canal. In rats with injection of CB-HRP into both acupoint regions and ipsilateral celiac ganglion, the dendrites originated from the labeled motoneurons projected directly to the labeled sympathetic preganglionic neurons.

CONCLUSION:

Each of the ST, BL and GB meridians is innervated by a specific group of motoneurons in the spinal cord. The motoneurons form a column with distinct border in the ventral horn of spinal cord, and the dendritic projections from the motoneurons oriented only to those innervating the same meridian. The dendrites from the meridian-related motoneurons can specifically project to the sympathetic preganglionic neurons at the thoracolumbar level.

• Authors: Ke Liu, Wan-Ru Duan, Chao Ma, Yi-Kuan Xie
• Journal: Zhen ci yan jiu = Acupuncture research (Zhen Ci Yan Jiu) Vol. 38 Issue 6 Pg. 447-52, 458 (Dec 2013) ISSN: 1000-0607 [Print] China
• PMID: 24579358 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Acupuncture Points
• Animals
• Dendrites (physiology)
• Female
• Ganglia, Sympathetic (cytology)
• Ganglion Cysts
• Humans
• Male
• Meridians
• Motor Neurons (cytology)
• Rats
• Rats, Sprague-Dawley
• Spinal Cord (cytology)