Evaluation of mouse experimental autoimmune uveoretinitis by spectral domain optical coherence tomography.

Abstract

AIMS:

To evaluate the efficacy of spectral domain optical coherence tomography (SD-OCT) in monitoring the development of mouse experimental autoimmune uveoretinitis (EAU) as an animal model of endogenous uveitis, and to develop an OCT-based grading system for EAU severity.

METHODS:

C57BL/6 mice were immunised with human interphotoreceptor retinoid-binding protein (amino acid sequence 1-20) peptide and complete Freund's adjuvant to induce EAU. The development of EAU was monitored by SD-OCT serially throughout the disease course, and the images were graded from 1 to 4 and compared with the clinical and histopathological grades.

RESULTS:

SD-OCT images depicted retinal lamella structures including the inner segment/outer segment (IS/OS) line in normal mice. Retinal structural changes were observed on SD-OCT images in mice that developed EAU clinically scored as grade 1 or higher, which precisely corresponded to the pathological findings. The SD-OCT images of EAU were graded as follows: grade 1, a few infiltrating cells in the vitreous and retina; grade 2, increased vitreous cells, retinal vasculitis, and granulomatous lesion; grade 3, cell infiltration into the whole retina, disappearance of IS/OS line, and destruction of the retinal layer structure; and grade 4, disappearance of the outer retina. The SD-OCT grade of EAU based on these criteria correlated significantly with both the clinical grade (R(2)=0.282, p<0.005) and histopathological grade (R(2)=0.846, p<0.0001).

CONCLUSIONS:

SD-OCT is useful for evaluating the development and severity of mouse EAU. The SD-OCT scoring system we developed accurately reflects clinical and histopathological changes.

• Authors: Kohzou Harimoto, Masataka Ito, Yoko Karasawa, Yutaka Sakurai, Masaru Takeuchi
• Journal: The British journal of ophthalmology (Br J Ophthalmol) Vol. 98 Issue 6 Pg. 808-12 (Jun 2014) ISSN: 1468-2079 [Electronic] England
• PMID: 24574437 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Chemical References

• Eye Proteins
• Peptide Fragments
• Retinol-Binding Proteins
• interstitial retinol-binding protein

Topics

• Animals
• Autoimmune Diseases (diagnosis)
• Disease Models, Animal
• Eye Proteins
• Female
• Mice
• Mice, Inbred C57BL
• Peptide Fragments
• Retinitis (chemically induced, diagnosis)
• Retinol-Binding Proteins
• Tomography, Optical Coherence
• Uveitis (chemically induced, diagnosis)