Abstract | BACKGROUND: METHODS: Mice were randomly assigned to six groups (n = 6 per group): sham-vehicle group, sham-PD1 group, sham- zVAD-fmk group, LPS-vehicle group, LPS-PD1 group, and LPS-PD1-zVAD-fmk group. Mice were injected intratracheally with 3 mg/kg LPS or saline, followed 24 hours later by intravenous injection of 200 µg/mouse PD1 or vehicle. At the same time, some mice were also injected intraperitoneally with the pan- caspase inhibitor zVAD-fmk. Seventy-two hours after LPS challenge, samples of pulmonary tissue and bronchoalveolar lavage fluid were collected. Optical microscopy was used to examine pathological changes in lungs. Cellularity and protein concentration in bronchoalveolar lavage fluid were analyzed. Lung wet/dry ratios and myeloperoxidase activity were measured. Apoptosis of neutrophils in bronchoalveolar lavage fluid (BALF) was also evaluated by flow cytometry. RESULTS: Intratracheal instillation of LPS increased neutrophil counts, protein concentration in bronchoalveolar lavage fluid and myeloperoxidase activity, it induced lung histological injury and edema, and also suppressed apoptosis of neutrophils in BALF. Posttreatment with PD1 inhibited LPS-evoked changes in BALF neutrophil counts and protein concentration and lung myeloperoxidase activity, with the outcome of decreased pulmonary edema and histological injury. In addition, PD1 promoted apoptosis of neutrophils in BALF. The beneficial effects of PD1 were blocked by zVAD-fmk. CONCLUSION:
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Authors | Xingwang Li, Chunlai Li, Wandong Liang, Yuntian Bi, Maohua Chen, Sheng Dong |
Journal | Chinese medical journal
(Chin Med J (Engl))
Vol. 127
Issue 5
Pg. 810-4
( 2014)
ISSN: 2542-5641 [Electronic] China |
PMID | 24571867
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Lipopolysaccharides
- protectin D1
- Docosahexaenoic Acids
- Peroxidase
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Topics |
- Acute Lung Injury
(chemically induced, drug therapy, immunology)
- Animals
- Apoptosis
(drug effects)
- Docosahexaenoic Acids
(therapeutic use)
- Inflammation
(drug therapy)
- Lipopolysaccharides
(toxicity)
- Male
- Mice
- Mice, Inbred BALB C
- Neutrophils
(cytology, drug effects)
- Peroxidase
(metabolism)
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