Abstract |
Increasing evidences in animals and humans suggest that CD8(+) T cells contribute significantly to immune defenses against Mycobacterium tuberculosis (Mtb). In the present study, we found that without any stimulation, CD8(+) T cells in pleural fluid cells (PFCs) expressed significantly higher levels of CD69 than PBMCs from patients with tuberculous pleurisy ( TBP). CD8(+)CD69(+) T cells expressed significantly higher levels of CD45RO and HLA-DR and lower levels of CD45RA than CD8(+)CD69(-) T cells, demonstrating that CD8(+)CD69(+) T cells were activated memory cells. Furthermore, we found higher expression of CCR6 and lower expression of CCR7 and CD62L on CD8(+)CD69(+) T cells compared with CD8(+)CD69(-) T cells, suggesting that the expression of CCR6 and reduced expression of CCR7 and CD62L might facilitate the migration of circulating CD8(+)CD69(+) T cells into tuberculous pleural space. Importantly, following stimulation with culture filtrate protein of 10 kDa (CFP10) peptides, CD8(+)CD69(+) T cells but not CD8(+)CD69(-) T cells expressed CD107a/b, IFN-γ and TNF-α, demonstrating that CD8(+)CD69(+) T cells were MTB-specific cells. In addition, the majority of CD8(+)CD69(+) T cells were dominated by polyfunctional T cells. In summary, we demonstrated that CD69 as a useful marker for MTB-specific CD8(+) T cells in PFCs from patients with TBP enabled a direct ex vivo estimation of the quantity, as well as the quality, of MTB-specific CD8(+) responses.
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Authors | Li Li, Binyan Yang, Xianlan Zhang, Suihua Lao, Changyou Wu |
Journal | Tuberculosis (Edinburgh, Scotland)
(Tuberculosis (Edinb))
Vol. 94
Issue 3
Pg. 219-25
(May 2014)
ISSN: 1873-281X [Electronic] Scotland |
PMID | 24566284
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Ltd. All rights reserved. |
Chemical References |
- Antigens, CD
- Antigens, Differentiation, T-Lymphocyte
- Biomarkers
- CD69 antigen
- Lectins, C-Type
- Tumor Necrosis Factor-alpha
- Interferon-gamma
- Leukocyte Common Antigens
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Topics |
- Adult
- Aged
- Antigens, CD
(metabolism)
- Antigens, Differentiation, T-Lymphocyte
(metabolism)
- Biomarkers
(metabolism)
- CD8-Positive T-Lymphocytes
(metabolism)
- Female
- Humans
- Interferon-gamma
(biosynthesis)
- Lectins, C-Type
(metabolism)
- Leukocyte Common Antigens
(metabolism)
- Male
- Middle Aged
- Mycobacterium tuberculosis
(immunology)
- Tuberculosis, Pleural
(immunology)
- Tumor Necrosis Factor-alpha
(biosynthesis)
- Young Adult
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