HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Mycobacterium tuberculosis-specific polyfunctional cytotoxic CD8+ T cells express CD69.

Abstract
Increasing evidences in animals and humans suggest that CD8(+) T cells contribute significantly to immune defenses against Mycobacterium tuberculosis (Mtb). In the present study, we found that without any stimulation, CD8(+) T cells in pleural fluid cells (PFCs) expressed significantly higher levels of CD69 than PBMCs from patients with tuberculous pleurisy (TBP). CD8(+)CD69(+) T cells expressed significantly higher levels of CD45RO and HLA-DR and lower levels of CD45RA than CD8(+)CD69(-) T cells, demonstrating that CD8(+)CD69(+) T cells were activated memory cells. Furthermore, we found higher expression of CCR6 and lower expression of CCR7 and CD62L on CD8(+)CD69(+) T cells compared with CD8(+)CD69(-) T cells, suggesting that the expression of CCR6 and reduced expression of CCR7 and CD62L might facilitate the migration of circulating CD8(+)CD69(+) T cells into tuberculous pleural space. Importantly, following stimulation with culture filtrate protein of 10 kDa (CFP10) peptides, CD8(+)CD69(+) T cells but not CD8(+)CD69(-) T cells expressed CD107a/b, IFN-γ and TNF-α, demonstrating that CD8(+)CD69(+) T cells were MTB-specific cells. In addition, the majority of CD8(+)CD69(+) T cells were dominated by polyfunctional T cells. In summary, we demonstrated that CD69 as a useful marker for MTB-specific CD8(+) T cells in PFCs from patients with TBP enabled a direct ex vivo estimation of the quantity, as well as the quality, of MTB-specific CD8(+) responses.
AuthorsLi Li, Binyan Yang, Xianlan Zhang, Suihua Lao, Changyou Wu
JournalTuberculosis (Edinburgh, Scotland) (Tuberculosis (Edinb)) Vol. 94 Issue 3 Pg. 219-25 (May 2014) ISSN: 1873-281X [Electronic] Scotland
PMID24566284 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014 Elsevier Ltd. All rights reserved.
Chemical References
  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • Biomarkers
  • CD69 antigen
  • Lectins, C-Type
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma
  • Leukocyte Common Antigens
Topics
  • Adult
  • Aged
  • Antigens, CD (metabolism)
  • Antigens, Differentiation, T-Lymphocyte (metabolism)
  • Biomarkers (metabolism)
  • CD8-Positive T-Lymphocytes (metabolism)
  • Female
  • Humans
  • Interferon-gamma (biosynthesis)
  • Lectins, C-Type (metabolism)
  • Leukocyte Common Antigens (metabolism)
  • Male
  • Middle Aged
  • Mycobacterium tuberculosis (immunology)
  • Tuberculosis, Pleural (immunology)
  • Tumor Necrosis Factor-alpha (biosynthesis)
  • Young Adult

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: