Formulated extract from multiple citrus peels impairs dendritic cell functions and attenuates allergic contact hypersensitivity.

Abstract

It has been reported that gold lotion (GL), a formulated product made from the peels of six citrus fruits, has many pharmacological properties, such as anti-tumor, antioxidant, and anti-inflammatory activities. In this study, we investigated the immunomodulatory effect of GL on lipopolysaccharide (LPS) stimulated mouse bone marrow-derived DC maturation and function. Our experimental results have shown that GL significantly impaired the pro-inflammatory cytokine and chemokine secretion, suppressed the expression of major histocompatibility complex class I/II and costimulatory molecules (CD40, CD80 and CD86), increased phagocytic capacity, and reduced propensity to stimulate the autologous CD4(+) and CD8(+) T cell proliferation of LPS-induced DCs. Furthermore, we found that oral administration of GL attenuated the 2,4-Dinitro-1-fluorobenzene induced contact hypersensitivity (CHS) in animal models. Subsequently, our molecular mechanism studies showed that GL interfered with LPS-induced MAPK-JNK, p38 phosphorylation and nuclear translocation of NF-κB p65. In an essence, these findings are the first report to provide new insight in the immunopharmacological role of GL in terms of its effects on DC.

• Authors: Shiming Li, Yi-Chin Lin, Chi-Tang Ho, Ping-Yi Lin, Michiko Suzawa, Hsin-Chieh Wang, Ching-Liang Chu, Der-Yuan Chen, Chi-Chen Lin
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 20 Issue 1 Pg. 12-23 (May 2014) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 24566093 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Cytokines
• Haptens
• Immunologic Factors
• Lipopolysaccharides
• NF-kappa B
• Plant Preparations
• Dinitrofluorobenzene
• Mitogen-Activated Protein Kinases

Topics

• Animals
• Bone Marrow Cells (cytology)
• Cell Differentiation (drug effects)
• Cells, Cultured
• Citrus
• Cytokines (immunology)
• Dendritic Cells (drug effects, physiology)
• Dermatitis, Allergic Contact (drug therapy, immunology)
• Dinitrofluorobenzene
• Female
• Haptens
• Immunologic Factors (pharmacology, therapeutic use)
• Lipopolysaccharides
• Mice, Inbred C57BL
• Mitogen-Activated Protein Kinases (metabolism)
• NF-kappa B (metabolism)
• Phagocytosis (drug effects)
• Phytotherapy
• Plant Preparations (pharmacology, therapeutic use)
• T-Lymphocytes (drug effects, immunology)