Abstract | BACKGROUND: RESULTS: The immunohistochemical expression of GSH, GSH-Px and GSTpi was compared with the clinical and pathological characteristics and the clinical outcome in the bitches, including metastasis and death.The results showed that high immunoexpression of GSH was correlated to the absence of tumor ulceration and was present in dogs without metastasis (P < 0.05). There was significant correlation of survival with the increase of GSH (P < 0.05). The expression of the GSH-Px and GSTpi enzymes showed no statistically significant correlation with the analyzed variables (p > 0.05). The analysis of the relative expression of genes responsible for the synthesis of GSH (GCLC and GSS) and GSH-Px by quantitative PCR was done with cultured cells of 10 tumor fragments from dogs with mammary tumors.The culture cells showed a decrease in GCLC and GSS expression when compared with no treated cells (P < 0.05). High GSH immunoexpression was associated with better clinical outcomes. CONCLUSION: Therefore, high expression of the GSH seems to play an important role in the clinical outcome of patients with mammary tumors and suggest its use as prognostic marker. The in vitro doxorubicin treatment significantly reduces the expression of GCLC and GSS genes so we can consider them to be candidates for predictive markers of therapeutic response in mammary cancer.
|
Authors | Camila Leonel, Gabriela B Gelaleti, Bruna V Jardim, Marina G Moschetta, Vitor R Regiani, Juliana G Oliveira, Debora Apc Zuccari |
Journal | BMC veterinary research
(BMC Vet Res)
Vol. 10
Pg. 49
(Feb 24 2014)
ISSN: 1746-6148 [Electronic] England |
PMID | 24565113
(Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antibiotics, Antineoplastic
- Biomarkers, Tumor
- Doxorubicin
- Glutathione Peroxidase
- Glutathione Transferase
- Glutathione
|
Topics |
- Animals
- Antibiotics, Antineoplastic
(therapeutic use)
- Biomarkers, Tumor
- Dog Diseases
(metabolism)
- Dogs
- Doxorubicin
(therapeutic use)
- Female
- Gene Expression Regulation, Neoplastic
(physiology)
- Glutathione
(metabolism)
- Glutathione Peroxidase
(genetics, metabolism)
- Glutathione Transferase
(genetics, metabolism)
- Mammary Neoplasms, Animal
(drug therapy, metabolism, pathology)
|