Abstract | OBJECTIVE: To investigate the proliferation inhibition effects of equol on human breast cancer cell line MCF-7 and explore its molecular mechanisms. METHODS: MCF-7 cells were treated with different concentration of equol, including 0.1, 0.5, 1, 5 and 10 micromol/L. After the treatment, the proliferation rate of cells was examined by MTT method and the cell apoptosis percentage and cell cycle phase were determined by flow cytometry. The bag-1, bcl-2, VEGF, ERK1/2, p-ERK1/2, p38 and p-p38 protein were determined using Western blotting. RESULTS: A dose and time effect of proliferation inhibition of equol was proved in MCF-7. The MCF-7 cell apoptosis percentage increased significantly in the groups of equol, and the cell cycle arrest in G0/G1 phase. The expression of bag-1, bcl-2, VEGF, p-ERK1/2 and p-p38 protein were decreased gradually with the increase concentration of equol. CONCLUSION: The equol could inhibit the proliferation of the breast cancer cell lines MCF-7 and its inhibitory effect may be due to inducing apoptosis, arresting the cell cycle in G0/G1 phase, down-regulating the expression of bag-1, bcl-2, VEGF, p-ERK1/2 and p-p38 protein.
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Authors | Meng Wang, Guofeng Ren |
Journal | Wei sheng yan jiu = Journal of hygiene research
(Wei Sheng Yan Jiu)
Vol. 43
Issue 1
Pg. 11-5
(Jan 2014)
ISSN: 1000-8020 [Print] China |
PMID | 24564104
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- BCL2-associated athanogene 1 protein
- DNA-Binding Proteins
- Proto-Oncogene Proteins c-bcl-2
- Transcription Factors
- VEGFA protein, human
- Vascular Endothelial Growth Factor A
- Equol
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Topics |
- Apoptosis
(drug effects)
- Cell Proliferation
(drug effects)
- DNA-Binding Proteins
(metabolism)
- Down-Regulation
(drug effects)
- Equol
(pharmacology)
- Female
- Humans
- MAP Kinase Signaling System
- MCF-7 Cells
- Proto-Oncogene Proteins c-bcl-2
(metabolism)
- Transcription Factors
(metabolism)
- Vascular Endothelial Growth Factor A
(metabolism)
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