Human beta 2-microglobulin specifically enhances cell-surface expression of HLA class I molecules in transfected murine cells.

Abstract

Sequential transfections of P815 murine mastocytoma cells with class I gene encoding either HLA-Cw3, HLA-A3, or HLA-B7 H chain and subsequently with a human beta 2-microglobulin gene were performed to evaluate the relative efficiency of human and murine beta 2-microglobulins in promoting the cell-surface expression of HLA-class I molecules. A 6-, 11-, and 40-fold specific enhancement of the cell-surface expression of HLA-Cw3, HLA-A3, and HLA-B7 molecules, respectively, was observed in cells co-transfected with human beta 2-microglobulin gene. This effect was attributed to a more efficient association of HLA H chains with human than with murine beta 2-microglobulin, which apparently allowed a more rapid transport of the HLA molecules from the endoplasmic reticulum to the Golgi apparatus.

• Authors: B M Perarnau, A C Gillet, R Hakem, M Barad, F A Lemonnier
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 141 Issue 4 Pg. 1383-9 (Aug 15 1988) ISSN: 0022-1767 [Print] United States
• PMID: 2456352 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Surface
• Epitopes
• HLA Antigens
• HLA-B7 Antigen
• Immunoglobulin Heavy Chains
• beta 2-Microglobulin
• RNA

Topics

• Animals
• Antigens, Surface (genetics, metabolism)
• Cell Line
• Epitopes
• Fluorescent Antibody Technique
• HLA Antigens (genetics, isolation & purification, metabolism)
• HLA-B7 Antigen
• Humans
• Immunoglobulin Heavy Chains (isolation & purification)
• Mice
• Precipitin Tests
• Protein Processing, Post-Translational
• RNA (isolation & purification)
• Transfection
• beta 2-Microglobulin (genetics, isolation & purification, physiology)