Abstract | BACKGROUND: Gymnema sylvestre is a highly valued ethno pharmacologically important medicinal plant used currently in many poly-herbal formulations due to its potential antidiabetic activity and other health benefits. The present study was carried out to analyze the anti-stress, anti-allergic, and antiulcer activity of the bioactive compounds present in Gymnema sylvestre leaves. METHODS: RESULTS: The quantitative estimation for aqueous extract exhibited total antioxidant (9.13 ± 0.04 μg/g), flavonoids (125.62 ± 26.84 μg/g), tannin (111.53 ± 15.13 μg/g), total phenol content (285.23 ± 1.11 μg/g) and free radical scavenging (52.14 ± 0.32%). Further the aqueous extract was consecutively purified by TLC and silica column chromatography. The purified fractions were characterized by HPTLC and GC-MS and the component was identified as gymnemic acid. The potency of the antimicrobial activity of the extract was studied with bacteria. Pharmacological experiments clearly demonstrated that the extracts of all plants given orally showed significant gastric protection against the asprin-induced gastric ulcer model in mice. Furthermore, healing effects were also confirmed through histopathological examination. CONCLUSIONS: The aqueous extracts of the leaves of Gymnema sylvestre possess anti ulcerogenic, Anti allergic, Anti stress, properties that may be due to cytoprotective mechanism. These results support the ethno medical uses of the plant in the treatment of gastric ulcer.
|
Authors | Lilly Baptista Arun, Aarrthy M Arunachalam, Kantha Deivi Arunachalam, Sathesh Kumar Annamalai, Kalaivani Amit Kumar |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 14
Pg. 70
(Feb 22 2014)
ISSN: 1472-6882 [Electronic] England |
PMID | 24559073
(Publication Type: Journal Article)
|
Chemical References |
- Anti-Allergic Agents
- Anti-Bacterial Agents
- Anti-Ulcer Agents
- Antioxidants
- Plant Extracts
- Polyphenols
- Saponins
- Tannins
- Triterpenes
- gymnemic acid
- Aspirin
|
Topics |
- Animals
- Anti-Allergic Agents
(isolation & purification, pharmacology, therapeutic use)
- Anti-Bacterial Agents
(isolation & purification, pharmacology, therapeutic use)
- Anti-Ulcer Agents
(isolation & purification, pharmacology, therapeutic use)
- Antioxidants
(isolation & purification, pharmacology, therapeutic use)
- Aspirin
- Chromatography, Thin Layer
- Eosinophilia
(chemically induced, drug therapy)
- Gymnema sylvestre
(chemistry)
- Male
- Mice
- Milk
- Phytotherapy
- Plant Extracts
(chemistry, pharmacology)
- Plant Leaves
- Polyphenols
(analysis)
- Saponins
(isolation & purification, pharmacology, therapeutic use)
- Stomach Ulcer
(chemically induced, drug therapy, pathology)
- Stress, Physiological
(drug effects)
- Tannins
(analysis)
- Triterpenes
(isolation & purification, pharmacology, therapeutic use)
|