Abstract |
Response surface methodology based on a central composite rotatable design has been successfully used to model and optimize biochemical and biotechnological processes. Heat treatment could have an effect on beta-lactoglobulin (β-Lg) and lead to allergic reaction. To reduce this phenomenon, liposomes were used as carriers in this research. The mass ratio of phosphatidylcholine and cholesterol (2.5-12.5), β-Lg concentration (2.5-12.5 mg mL(-1)), sonication time (5-25, min) and temperature (25-40 °C) were selected as independent variables with encapsulation efficiency as a dependent variable. For each response, a second-order polynomial model was developed using multiple linear regression analysis. Applying a desirability function method the optimum parameters were: phosphatidylcholine to cholesterol ratio of 8.05, β-Lg concentration of 9.09 mg mL(-1), sonication time of 17.71 min and temperature of 30 °C. The particle size and encapsulation efficiency were found to be 189 nm and 61.55%, respectively. Furthermore, the in vitro stability of β-Lg nano- liposomes in simulated gastrointestinal juice was evaluated. The nano- liposomes showed an acceptable stability in simulated gastrointestinal juice at 37 °C for 4 h.
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Authors | Jieqing Ma, Rongfa Guan, Xiaoqiang Chen, Yanbo Wang, Yunbin Hao, Xingqian Ye, Mingqi Liu |
Journal | Food & function
(Food Funct)
Vol. 5
Issue 4
Pg. 748-54
(Apr 2014)
ISSN: 2042-650X [Electronic] England |
PMID | 24554141
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Drug Carriers
- Lactoglobulins
- Liposomes
- Phosphatidylcholines
- Cholesterol
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Topics |
- Cholesterol
(chemistry)
- Drug Carriers
(chemistry)
- Drug Compounding
(methods)
- Lactoglobulins
(chemistry)
- Liposomes
(chemistry)
- Nanoparticles
(chemistry)
- Particle Size
- Phosphatidylcholines
(chemistry)
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