microRNA-155 acts as an oncogene by targeting the tumor protein 53-induced nuclear protein 1 in esophageal squamous cell carcinoma.

Abstract	MicroRNA-155 (miR-155) is overexpressed in many human cancers; however, the function of miR-155 is largely unknown in esophageal squamous cell carcinoma (ESCC). In the present study, we found that miR-155 is dramatically increased in ESCC tissues compared with the paired adjacent normal tissues, which suggested that miR-155 acts as an oncogene in ESCC. We predicted that tumor protein p53-induced nuclear protein 1 (TP53INP1) is a candidate target gene of miR-155 given that miR-155 expression decreased mRNA and protein levels of TP53INP1 as determined by RT-PCR and Western blot analysis. In addition, miR-155 and TP53INP1 showed a negative relation in ESCC tissues. Dual luciferase-based reporter assay indicated direct regulation of TP53INP1 by miR-155. Furthermore, we demonstrated that RNA interference of TP53INP1 increased the proliferation and colonies formation of EC-1 cells. Up-regulation of TP53INP1 abrogated miR-155 induced growth in EC-1 cells and mutation of TP53INP1 in 3'-UTR restored the effects when co-transfected with miR-155. We also indicated that overexpression of miR-155 significantly promoted the proliferation of EC-1 cells in vitro and the development of tumors in nude mice. Taken together, our study reveals that miR-155 acts as an oncogene by targeting TP53INP1 in ESCC.
Authors	Jie Zhang, Chen Cheng, Xiang Yuan, Jiang-Tu He, Qiu-Hui Pan, Fen-Yong Sun
Journal	International journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 7 Issue 2 Pg. 602-10 ( 2014) ISSN: 1936-2625 [Electronic] United States
PMID	24551280 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	3' Untranslated Regions Carrier Proteins Heat-Shock Proteins MIRN155 microRNA, human MicroRNAs TP53INP1 protein, human
Topics	3' Untranslated Regions Animals Carcinoma, Squamous Cell (genetics, metabolism, pathology) Carrier Proteins (genetics, metabolism) Cell Line, Tumor Cell Proliferation Esophageal Neoplasms (genetics, metabolism, pathology) Esophageal Squamous Cell Carcinoma Female Gene Expression Regulation, Neoplastic Heat-Shock Proteins (genetics, metabolism) Humans Male Mice Mice, Nude MicroRNAs (genetics, metabolism) Middle Aged Oncogenes RNA Interference Time Factors Transfection Tumor Burden Up-Regulation

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