Abstract | OBJECTIVE: MATERIALS AND METHODS: Cytotoxicity was determined with killing ability of NK cells against yeast artificial chromosome (YAC)-1 cells. Interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) productions by NK cells were measured by enzyme-linked immunosorbent assay (ELISA). Proliferations of NK and Meth A cells were determined by [(3)H] thymidine incorporated proliferation and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) methods, respectively. RESULTS: KLH at 6.25, 12.5, and 25 μg/well augmented cytotoxicity of NK cells against YAC-1 cells by 2.5, three, and five-times, respectively. KLH at 25 μg/well enhanced IFN-γ and TNF-α productions by 17- and 23-folds, respectively. The proliferation of NK cells was three times stimulated by KLH. The proliferation of Meth A cells was markedly inhibited by all the doses; the highest (4-folds higher) inhibition was observed at a dose of KLH (25 μg/well). CONCLUSION: The study demonstrated the anticancer activity of KLH acting through the induction of NK cells and inhibition of cancer cells. KLH, therefore, may be a good candidate for an anticancer agent alone or in combination with other chemotherapeutic agents.
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Authors | Md Moklesur Rahman Sarker, Ming Zhong |
Journal | Indian journal of pharmacology
(Indian J Pharmacol)
2014 Jan-Feb
Vol. 46
Issue 1
Pg. 40-5
ISSN: 1998-3751 [Electronic] India |
PMID | 24550583
(Publication Type: Journal Article)
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Chemical References |
- Cytokines
- Hemocyanins
- keyhole-limpet hemocyanin
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Topics |
- Animals
- Cell Death
(drug effects)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cytokines
(biosynthesis)
- Female
- Hemocyanins
(pharmacology)
- Killer Cells, Natural
(cytology, drug effects, metabolism)
- Mice
- Mice, Inbred C57BL
- Sarcoma, Experimental
(pathology)
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