A glioma classification scheme based on coexpression modules of EGFR and PDGFRA.

Abstract

We hypothesized that key signaling pathways of glioma genesis might enable the molecular classification of gliomas. Gene coexpression modules around epidermal growth factor receptor (EGFR) (EM, 29 genes) or platelet derived growth factor receptor A (PDGFRA) (PM, 40 genes) in gliomas were identified. Based on EM and PM expression signatures, nonnegative matrix factorization reproducibly clustered 1,369 adult diffuse gliomas WHO grades II-IV from four independent databases generated in three continents, into the subtypes (EM, PM and EM(low)PM(low) gliomas) in a morphology-independent manner. Besides their distinct patterns of genomic alterations, EM gliomas were associated with higher age at diagnosis, poorer prognosis, and stronger expression of neural stem cell and astrogenesis genes. Both PM and EM(low)PM(low) gliomas were associated with younger age at diagnosis and better prognosis. PM gliomas were enriched in the expression of oligodendrogenesis genes, whereas EM(low)PM(low) gliomas were enriched in the signatures of mature neurons and oligodendrocytes. The EM/PM-based molecular classification scheme is applicable to adult low-grade and high-grade diffuse gliomas, and outperforms existing classification schemes in assigning diffuse gliomas to subtypes with distinct transcriptomic and genomic profiles. The majority of the EM/PM classifiers, including regulators of glial fate decisions, have not been extensively studied in glioma biology. Subsets of these classifiers were coexpressed in mouse glial precursor cells, and frequently amplified or lost in an EM/PM glioma subtype-specific manner, resulting in somatic copy number alteration-dependent gene expression that contributes to EM/PM signatures in glioma samples. EM/PM-based molecular classification provides a molecular diagnostic framework to expedite the search for new glioma therapeutic targets.

• Authors: Yingyu Sun, Wei Zhang, Dongfeng Chen, Yuhong Lv, Junxiong Zheng, Henrik Lilljebjörn, Liang Ran, Zhaoshi Bao, Charlotte Soneson, Hans Olov Sjögren, Leif G Salford, Jianguang Ji, Pim J French, Thoas Fioretos, Tao Jiang, Xiaolong Fan
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 111 Issue 9 Pg. 3538-43 (Mar 04 2014) ISSN: 1091-6490 [Electronic] United States
• PMID: 24550449 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ErbB Receptors
• Receptor, Platelet-Derived Growth Factor alpha

Topics

• Age Factors
• Animals
• China
• Cluster Analysis
• ErbB Receptors (genetics, metabolism)
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic (genetics)
• Glioma (classification, genetics)
• Humans
• Kaplan-Meier Estimate
• Mice
• Neural Stem Cells (metabolism)
• Polymorphism, Single Nucleotide (genetics)
• Prognosis
• Receptor, Platelet-Derived Growth Factor alpha (genetics, metabolism)
• Signal Transduction (genetics)