Abstract |
Iso- suillin, an isomer of suillin that belongs to the prenylphenol class of fungal derivatives, was isolated from petroleum ether extracts of Suillus flavus. The IC50 value of iso- suillin in K562 cells was 0.87 μM, which was lower than the positive control cisplatin (19.33 μM). Iso- suillin-treated K562 cells exhibited an increased rate of apoptosis, mitochondrial membrane potential ( MMP) depolarization, and G0/G1 arrest. Western blot analysis revealed that these cells displayed significantly upregulated expression of several apoptosis-related proteins, including cytochrome c, caspase 9, FADD (Fas-associating protein with a novel death domain), caspase 8, caspase 3, and Bax. Moreover, the expression of two anti-apoptosis proteins, NF-κB and Bcl-2, was downregulated. Inhibitors of caspase 9 and caspase 8 protected the K562 cells from apoptosis. Taken together, our results suggest that iso- suillin induces K562 apoptosis through the mitochondrial and death receptor pathways and that iso- suillin may represent a candidate anti- leukemia treatment.
|
Authors | Ying Wang, Qingshuang Zhang, Junxia Zhao, Xiaojing Zhao, Jinxiu Zhang, Li-an Wang |
Journal | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
(Food Chem Toxicol)
Vol. 67
Pg. 17-25
(May 2014)
ISSN: 1873-6351 [Electronic] England |
PMID | 24534064
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2014 Elsevier Ltd. All rights reserved. |
Chemical References |
- Diterpenes
- Phenols
- suillin
|
Topics |
- Agaricales
(chemistry)
- Apoptosis
(drug effects)
- Cell Cycle
(drug effects)
- Diterpenes
- Humans
- K562 Cells
- Membrane Potential, Mitochondrial
(drug effects)
- Phenols
|