Coxiella burnetii serology assays in goat abortion storm.

Abstract

Many commercial antibody detection enzyme-linked immunosorbent assay (ELISA) kits for Q fever utilize the Nine Mile (Montana tick) strain of Coxiella burnetii as antigen. An ELISA kit manufactured in France employs ovine placenta-sourced antigen and has been used in Europe. Sera from goats experiencing a Q fever abortion storm in the United States were used to compare the sensitivity and specificity of these 2 ELISA formats and the Q fever complement fixation test (CFT). Latent class estimates of sensitivity ranged from 97% to 100% with a specificity of 95-100% for the 2 ELISA kits. Estimates for sensitivity and specificity of the CFT were 89% and 82%, respectively. There was not a significant increase in ELISA sensitivity observed with the ovine-sourced antigen kit in this study. Real-time polymerase chain reactions performed on a portion of the sera found that 15 out of 20 sera were congruent across 4 tests for positive and negative sera.

• Authors: Michelle P Emery, Eileen N Ostlund, Mohamed Ait Ichou, Jeff D Ballin, David McFarling, Luanne McGonigle
• Journal: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (J Vet Diagn Invest) Vol. 26 Issue 1 Pg. 141-5 (Jan 2014) ISSN: 1943-4936 [Electronic] United States
• PMID: 24532695 (Publication Type: Journal Article)

Chemical References

• Antibodies, Bacterial
• DNA, Bacterial

Topics

• Abortion, Veterinary (microbiology)
• Animals
• Antibodies, Bacterial (blood)
• Complement Fixation Tests (veterinary)
• Coxiella burnetii (isolation & purification)
• DNA, Bacterial (chemistry, genetics)
• Disease Outbreaks (veterinary)
• Enzyme-Linked Immunosorbent Assay (veterinary)
• Female
• Goat Diseases (blood, microbiology)
• Goats
• Polymerase Chain Reaction (veterinary)
• Pregnancy
• Q Fever (blood, microbiology, veterinary)
• Sensitivity and Specificity
• United States